LOCUS       sequence-117327-        4081 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Middle entry clone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Shin M, Male I, Beane TJ, Villefranc JA, Kok FO, Zhu LJ, Lawson ND
  TITLE     Vegfc acts through ERK to induce sprouting and differentiation of 
            trunk lymphatic progenitors.
  JOURNAL   Development. 2016 Oct 15;143(20):3785-3795. Epub 2016 Sep 12.
  PUBMED    27621059
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3785-3795. Epub 2016 Sep 12"
COMMENT     Sequence Label: pME-6MTzactMap2k2b
FEATURES             Location/Qualifiers
     source          1..4081
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             713..742
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             752..781
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             791..820
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             830..859
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             869..898
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             932..961
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(2208..2307)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(2325..2343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2348..2364)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2348..2364)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2466..2485)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2477..3286
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2544..2563)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3433..4021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3922..3941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gccaccatgc tttttgcagg atcccatcga tttaaagcta tggagcaaaa
      721 gctcatttct gaagaggact tgaatgaaat ggagcaaaag ctcatttctg aagaggactt
      781 gaatgaaatg gagcaaaagc tcatttctga agaggacttg aatgaaatgg agcaaaagct
      841 catttctgaa gaggacttga atgaaatgga gcaaaagctc atttctgaag aggacttgaa
      901 tgaaatggag agcttgggcg acctcaccat ggagcaaaag ctcatttctg aagaggactt
      961 gaatttagag atctcgaatt caaggcctct cgagatggct cccaaaaaga ggccagatcc
     1021 tatagttata actggaagcg gagaagcaca gacgacctcc acgcccatag atgctgcatc
     1081 agaagcgaat cttgaagccc tgcagaagaa gctggatgag ctggatttgg acgagcagca
     1141 gaagaagcgt ctggaagctt tcctcacgca gaaagccaat ctgggagagc tgaaggacga
     1201 tgatttccag catatctgtg aactgggagc tggaaacgga ggagtggtga ataaagtgtg
     1261 ccacaaacct tcaggtctcg tcatggcccg gaagttaatt cacctggaga tcaagccggc
     1321 catcaggcat cagatcatca gagagctgca ggtcttgcat gagtgtaatt caccatacat
     1381 cgtgggtttt tacggcgcat tttacagcga tggcgagatt agtatctgta tggagaacat
     1441 ggacggcgga tcgttggatc aggtcctgaa agaggctcgc agaatcccag aggagatttt
     1501 gggaaaagtc agcatagctg ttttaagagg tcttgcttac cttcgagaaa agcaccagat
     1561 tattcacaga gatgtgaaac cctcaaatat tctggtaaac tgtcgcgggg agatcaagct
     1621 gtgtgacttc ggtgtgagcg ggcagctgat cgacgacatg gccaacgact ttgtaggaac
     1681 acgctcatac atgtcgccgg agcggttgca gggcactcat tactcggttc agtcagacgt
     1741 gtggagtatg ggtctctctc tggtggagct cgccatcggc cgatacccta ttcctccacc
     1801 tgacgccaag gagctggagg gcatcttcgg cagggcactg atggatgttg gagattcaga
     1861 aacacacagc acgtctcctc gacccagacc acctggcaga cccatcagcg gtcatgggcc
     1921 ggtcatggct atatttgagc tgctggatta tatagtcaat gagcctcctc ctaaactgcc
     1981 tcacggcgtc ttcactcctg atttccagga ctttgtgacc aactgtctta ttaaaaaccc
     2041 tgcagacaga gcggacctga agatgctgat gaatcacaca tttatcaaac gcgccgaagt
     2101 ggaggagatg gactttgctg gatggttgtg taaaacaatg ggtctcaatc agcccagcac
     2161 acccacacaa acaactgaat ctagaactat agtgagtcgt attacgcacc cagctttctt
     2221 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     2281 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     2341 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     2401 cattgcacaa gataaaataa tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2461 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2521 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2581 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2641 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2701 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2761 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2821 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2881 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2941 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     3001 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     3061 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     3121 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     3181 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     3241 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     3301 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     3361 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3421 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3481 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3541 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     3601 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3661 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3721 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3781 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     3841 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3901 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3961 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     4021 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     4081 g
//
