LOCUS       sequence-117331-         977 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Doxycycline-regulated mammalian expression vector for expressing 
            shRNA against E2F1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 977)
  AUTHORS   Hoog G, Zarrizi R, von Stedingk K, Jonsson K, Alvarado-Kristensson M
  TITLE     Nuclear localization of gamma-tubulin affects E2F transcriptional 
            activity and S-phase progression.
  JOURNAL   FASEB J. 2011 Nov;25(11):3815-27. doi: 10.1096/fj.11-187484. Epub 
            2011 Jul 25.
  PUBMED    21788450
REFERENCE   2  (bases 1 to 977)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1096/fj.11-187484"; journalName: "FASEB J"; date: "2011-11"; 
            volume: "25"; issue: "11"; pages: "3815-27"
COMMENT     Sequence Label: pTER E2F1shRNA human
FEATURES             Location/Qualifiers
     source          1..977
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(207..226)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        428..532
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(751..770)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gactcgagcg gccgccactg tgctggatat ctgcagaatt ccaccacact ggactagtgg
       61 atccgagctc ggtaccaagc ttttccaaaa atatctgtac tacgcagctg tctcttgaac
      121 agctgcgtag tacagatagg gatctctatc actgataggg aacttataag attcccaaat
      181 ccaaagacat ttcacgttta tggtgatttc ccagaacaca tagcgacatg caaatattgc
      241 agggcgccac tcccctgtcc ctcacagcca tcttcctgcc agggcgcacg cgcgctgggt
      301 gttcccgcct agtgacactg ggcccgcgat tccttggagc gggttgatga cgtcagcgtt
      361 cgaattcctg cagcccgggg gatctcccga tccgtcgacg tcaggtggca cttttcgggg
      421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
      781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
      841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
      901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
      961 gaaggagcta accgctt
//
