LOCUS       sequence-117333-        1090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: F2R-Tango
FEATURES             Location/Qualifiers
     source          1..1090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatggg cccgcggcgc cttttgcttg tcgcggcatg cttttctctc
      301 tgtggtccac tgcttagcgc ccggacccgc gctcgcaggc cggagtctaa agccacgaat
      361 gccaccctgg atccaaggtc atttctcctc aggaatccaa acgataaata cgagccgttt
      421 tgggaagatg aagagaagaa cgagagtggg cttaccgaat atcgcctggt atctataaat
      481 aagagctccc cgctccagaa gcagttgccc gctttcatta gtgaggacgc cagtggatat
      541 cttacatcta gttggttgac tctgtttgtc ccgagcgttt atacaggggt ctttgtggtt
      601 tccctgccac tgaacatcat ggccatcgtc gtgttcattc tgaagatgaa ggtgaaaaaa
      661 cctgctgtcg tatatatgct tcacctggca acggccgacg tgctctttgt gagcgtgctg
      721 ccctttaaga taagctacta cttttccgga agcgattggc agttcggaag cgagctgtgc
      781 cgcttcgtta ctgccgcctt ttattgcaac atgtacgcct ccatcctcct gatgaccgtg
      841 attagcatcg accgattcct cgcagtcgta tatccaatgc agtcactgtc ctggcgcaca
      901 ctgggtcgcg catcttttac atgcctggca atatgggcgc ttgccatcgc cggcgtggtg
      961 cccctgctgc tgaaggagca gactatccag gttcccgggc tgaacataac cacctgtcac
     1021 gatgtgctca acgaaaccct tctcgaaggc tattacgcgt actatttctc cgcgttcagc
     1081 gctgtttttt
//
