LOCUS       sequence-117353-         700 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans 
            K08F4.2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Paix A, Wang Y, Smith HE, Lee CY, Calidas D, Lu T, Smith J, Schmidt 
            H, Krause MW, Seydoux G
  TITLE     Scalable and versatile genome editing using linear DNAs with 
            microhomology to Cas9 Sites in Caenorhabditis elegans.
  JOURNAL   Genetics. 2014 Dec;198(4):1347-56. doi: 10.1534/genetics.114.170423.
            Epub 2014 Sep 23.
  PUBMED    25249454
REFERENCE   2  (bases 1 to 700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.114.170423"; journalName: "Genetics"; date: 
            "2014-12"; volume: "198"; issue: "4"; pages: "1347-56"
COMMENT     Sequence Label: AP324-2
FEATURES             Location/Qualifiers
     source          1..700
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(51..73)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_RNA        complement(345..420)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
       61 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      121 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
      181 gagcagattg cactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
      241 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
      301 tcggtgcggg cctcttcgct attacgccag aaatttcaca aaaagcaccg actcggtgcc
      361 actttttcaa gttgataacg gactagcctt attttaactt gctatttcta gctctaaaac
      421 cattttggaa accgctgcgc aagacatctc gcaataggag gtgtttatat aggggcaaca
      481 caagaggcgg cagacatctc gtgagtttga cagagtagta taaagagtgt gtgtgagagg
      541 gtgtaggcat ttcataacat attttttgtg gaataatttt ggaaaaaagg tgttttttta
      601 ataatagctt ttataagagt ttctcaaaaa ttgcatgctt tttttgaaaa ggttgacaaa
      661 ttatagtagt actaaagttg ctattttcag gtctcagcag
//
