LOCUS       sequence-117367-         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively express mitochondrial localized PhyB in Saccharomyces
            cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Jost AP, Weiner OD
  TITLE     Probing Yeast Polarity with Acute, Reversible, Optogenetic 
            Inhibition of Protein Function.
  JOURNAL   ACS Synth Biol. 2015 Jun 2.
  PUBMED    26035630
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Jun 2."
COMMENT     Sequence Label: PhyB-mCherry-Tom7
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 taatacgact cactataggg ggccagcgtg gccgtttaaa catttaaatg gccggcctac
       61 gtcgttaagg ccgtttctga cagagtaaaa ttcttgaggg aactttcacc attatgggaa
      121 atggttcaag gaggtattga cttaaactcc atcaaatggt caggtcattg agtgtttttt
      181 atttgttgta tttttttttt tttagagaaa atcctccaat atataaatta ggaatcatag
      241 tttcatgatt ttctgttaca cctaactttt tgtgtggtgc cctcctcctt gtcaatatta
      301 atgttaaagt gcaattcttt ttccttatca cgttgagcca ttagtatcaa tttgcttacc
      361 tgtattcctt tacatcctcc tttttctcct tcttgataaa tgtatgtaga ttgcgtatat
      421 agtttcgtct accctatgaa catattccat tttgtaattt cgtgtcgttt ctattatgaa
      481 tttcatttat aaagtttatg tacaaatatc ataaaaaaag agaatctttt taagcaagga
      541 ttttcttaac ttcttcggcg acagcatcac cgacttcggt ggccacacaa ttataagcaa
      601 agggggtaca atcattacac cacaacacaa cacccagttt aatttccatg agttcaaatc
      661 aagagtggtc ccaagatata ttaaaacaat tgaatctacg agactctcgg gaaaaaaacc
      721 atgagattga tgctagatat ttcaacgcct tccagcaatt gtctcaaaaa ttgaatcaat
      781 tatccacaac aacaaatact cttctacagg accaatcaag caattcagaa actttgacaa
      841 atctaataaa ctctaaagaa attgaaaact tgaaacttga aaacaccgag ttgataacta
      901 acttaaataa tttaattatc aataatgaaa aactaacaca aacaatcaat gcc
//
