LOCUS       sequence-117389-         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CCRL2-Tango
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatggc caattatacg ctggcgcctg aggatgaata cgatgtgctg
      301 attgagggtg aactggaaag cgacgaagcc gaacaatgtg ataagtatga cgcacaggct
      361 ctctccgctc agcttgtccc cagcctctgt tcagccgtct ttgtgatcgg agtcctggac
      421 aatctgttgg tggtgctcat acttgtgaag tacaagggcc tgaagcgcgt cgagaacatt
      481 tacctgctga atctggctgt gtccaatctg tgttttctgc tgaccttgcc attctgggct
      541 cacgctgggg gcgacccaat gtgtaagatt ttgataggac tgtacttcgt gggactttat
      601 tctgagacgt ttttcaattg cttgctcacc gtccaacggt acctggtttt cctgcacaaa
      661 ggtaactttt tctctgccag gagacgagtg ccgtgcggta taattacaag cgttttggcg
      721 tgggttacgg cgatcctggc aaccctgccc gagttcgtgg tctataagcc tcaaatggag
      781 gatcaaaagt acaaatgcgc cttttctcgg acacctttcc ttccggcaga cgagaccttc
      841 tggaagcact ttcttaccct taaaatgaa
//
