LOCUS       sequence-117413-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase expression driven by miR-34a promoter P6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Chang TC, Wentzel EA, Kent OA, Ramachandran K, Mullendore M, Lee KH,
            Feldmann G, Yamakuchi M, Ferlito M, Lowenstein CJ, Arking DE, Beer 
            MA, Maitra A, Mendell JT
  TITLE     Transactivation of miR-34a by p53 broadly influences gene expression
            and promotes apoptosis.
  JOURNAL   Mol Cell. 2007 Jun 8;26(5):745-52. Epub 2007 May 31.
  PUBMED    17540599
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-8"; volume: "26(5)"; pages: "745-52. Epub 2007 May 
            31"
COMMENT     Sequence Label: pGL3-IRES-miR34a-P6
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      176..185
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(248..266)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tccccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc
       61 gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
      121 acgatgataa gatctgcgat ctaagtaagc ttggcattcc ggtactgttg gtaaagccac
      181 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg
      241 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat
      301 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc
      361 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt
      421 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt
      481 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat
      541 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca
      601 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg
      661 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga
      721 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg
      781 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc
      841 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt
      901 tgttccattc catcacg
//
