LOCUS       sequence-117420-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD283
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(421..726)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(523..542)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aatttaaata atcagggtta gttagtatat atatgtttaa acttaccttg aagaagatgg
       61 tacgctcttg gacgtatccc tctggcatgg cggacttgaa gaagtcgtgt tgcttcatgt
      121 ggtctgggta acgggcgaag cattggactc cgtatccgag ggtggtgacg agggttggcc
      181 atgggactgg gagctttccg gtggtgcaga ggagcttgag ggtgagcttt ccgtaggtgg
      241 cgtctccctc tccctctccg gagacggaga acttgtgtcc gttgacgtct ccgtcgagct
      301 cgacgaggat aggtacaact cctgtaaata actcttctcc ttttgacatc gatgctcctg
      361 aggctcccga tgctccctag gtcgacctgc agactggctg tgtataaggg agcctgacat
      421 ttatattccc cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag
      481 atcagccact tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat
      541 gcgccagctt tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga
      601 cagcagacgt gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc
      661 actctgtaca tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa
      721 ctgcatttca ccagcccctg ttctcgtcgg caaaagagcc g
//
