LOCUS       sequence-117421-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD283
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(144..168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(521..554)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             573..596
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             600..623
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             627..650
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(800..819)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
       61 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
      121 ctcgtccgag ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct
      181 tcaacatccc tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa
      241 aaaacttctc ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat
      301 tgtgtagatt caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct
      361 ccccccatta ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat
      421 gttttttact tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc
      481 ttatcatatg ttacgtttca gtttatgacc gcaattttta ataacttcgt atagcataca
      541 ttatacgaag ttattttcag ggagccggat ctgattataa agacgatgac gataagcgtg
      601 actacaagga cgacgacgac aagcgtgatt acaaggatga cgatgacaag agactagttc
      661 gacctgcaga ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt
      721 taatggcgtt tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa
      781 cggagaccgg cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat
      841 gcaccaccgg gtaaagttca c
//
