LOCUS       sequence-117424-         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD284
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(143..167)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(520..553)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             572..595
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             599..622
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             626..649
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(799..818)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 catcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc
       61 cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac
      121 tcgtccgagg gcaaaggaat agacccagct ttcttgtaca aagtgggtcc aattactctt
      181 caacatccct acatgctctt tctccctgtg ctcccacccc ctatttttgt tattatcaaa
      241 aaacttctct taatttcttt gttttttagc ttcttttaag tcacctctaa caatgaaatt
      301 gtgtagattc aaaaatagaa ttaattcgta ataaaaagtc gaaaaaaatt gtgctccctc
      361 cccccattaa taataattct atcccaaaat ctacacaatg ttctgtgtac acttcttatg
      421 ttttttactt ctgataaatt tttttgaaac atcatagaaa aaaccgcaca caaaatacct
      481 tatcatatgt tacgtttcag tttatgaccg caatttttaa taacttcgta tagcatacat
      541 tatacgaagt tattttcagg gagccggatc tgattataaa gacgatgacg ataagcgtga
      601 ctacaaggac gacgacgaca agcgtgatta caaggatgac gatgacaaga gactagttcg
      661 acctgcagac tggctgtgta taagggagcc tgacatttat attccccaga acatcaggtt
      721 aatggcgttt ttgatgtcat tttcgcggtg gctgagatca gccacttctt ccccgataac
      781 ggagaccggc acactggcca tatcggtggt catcatgcgc cagctttcat ccccgatatg
      841 caccacc
//
