LOCUS       sequence-117426-         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD285
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             499..519
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(537..570)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             580..609
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ggagtcctca ccgccaccca agacacctcc ctccaagacg gatgcctcat ctacaacgtc
       61 aaggtaagtt taaacagttc ggtactaact aaccatacat atttaaattt tcagatccgt
      121 ggagtcaact tcccatccaa cggaccagtc atgcaaaaga agaccctcgg atgggaggcc
      181 tccaccgaga ccctctaccc agccgacgga ggacttgagg gacgtgccga catggccctc
      241 aagctcgtcg gaggaggaca cctcatctgc aacctcaagg taagtttaaa catgatttta
      301 ctaactaact aatctgattt aaattttcag accacctacc gttccaagaa gccagccaag
      361 aacctcaaga tgccaggagt ctactacgtc gaccgtcgtc ttgagcgtat caaggaggcc
      421 gacaaggaga cctacgtcga gcaacacgag gtcgccgtcg cccgttactg cgacctccca
      481 tccaagctcg gacaccgtga gaatctgtac tttcaatccg gaaaggtaag tttaaaataa
      541 cttcgtatag catacattat acgaagttat tttcagggag agcaaaagct tatctccgag
      601 gaggacctct aaggatgatc gacgccaacg tcgttgaatt ttcaaatttt aaatactgaa
      661 tatttgtttt ttttcctatt atttatttat tctctttgtg ttttttttct tgctttctaa
      721 aaaattaatt caatccaaat ctaaacattt ttttttctct ttccgtctcc caattcgtat
      781 tccgctcctc tcatctgaac acaatgtgca agtttattta tcttctcgct ttcatttcat
      841 taggacgtgg ggggaattgg tggaaggggg aaacc
//
