LOCUS       sequence-117427-         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD285
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(280..585)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(382..401)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ttaccttgga tccgtacatg aaggaggtgg cgaggatgtc gaaggcgaat gggagtggtc
       61 ctccctcgac ggccttgata cgcatggttt gggttccctc gtatggcttt ccctctccct
      121 cggaggtgca cttgaagtgg tggttgttga cggttccctc catgtagagc ttcatatgca
      181 tgttttcttt aatgagctcg gagaccatcg atgctcctga ggctcccgat gctccctagg
      241 tcgacctgca gactggctgt gtataaggga gcctgacatt tatattcccc agaacatcag
      301 gttaatggcg tttttgatgt cattttcgcg gtggctgaga tcagccactt cttccccgat
      361 aacggagacc ggcacactgg ccatatcggt ggtcatcatg cgccagcttt catccccgat
      421 atgcaccacc gggtaaagtt cacgggggac tttatctgac agcagacgtg cactggccag
      481 ggggatcacc atccgtcgcc cgggcgtgtc aataatatca ctctgtacat ccacaaacag
      541 acgataacgg ctctctcttt tataggtgta aaccttaaac tgcatttcac cagcccctgt
      601 tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc gacctc
//
