LOCUS       sequence-117428-         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mKate2^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Dickinson DJ, Pani AM, Heppert JK, Higgins CD, Goldstein B
  TITLE     Streamlined Genome Engineering with a Self-Excising Drug Selection 
            Cassette.
  JOURNAL   Genetics. 2015 Jun 3. pii: genetics.115.178335.
  PUBMED    26044593
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2015 Jun 3. pii: genetics.115.178335."
COMMENT     Sequence Label: pDD285
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(134..158)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(511..544)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             563..586
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             590..613
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             617..640
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(790..809)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
       61 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
      121 ggcaaaggaa tagacccagc tttcttgtac aaagtgggtc caattactct tcaacatccc
      181 tacatgctct ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc
      241 ttaatttctt tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt
      301 caaaaataga attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta
      361 ataataattc tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact
      421 tctgataaat ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg
      481 ttacgtttca gtttatgacc gcaattttta ataacttcgt atagcataca ttatacgaag
      541 ttattttcag ggagccggat ctgattataa agacgatgac gataagcgtg actacaagga
      601 cgacgacgac aagcgtgatt acaaggatga cgatgacaag agactagttc gacctgcaga
      661 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      721 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      781 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      841 gtaaagttca cggggg
//
