LOCUS       sequence-117429-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Fiordalisi JJ, Johnson RL 2nd, Ulku AS, Der CJ, Cox AD
  TITLE     Mammalian expression vectors for Ras family proteins: generation and
            use of expression constructs to analyze Ras family function.
  JOURNAL   Methods Enzymol. 2001;332:3-36. doi: 10.1016/s0076-6879(01)32189-4.
  PUBMED    11305105
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods 
            Enzymol."; date: "2001"; volume: "332:3-36. doi"; pages: " 
            10.1016/s0076-6879(01)32189-4"
COMMENT     Sequence Label: pCGN R-Ras 38V, 61S
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     5'UTR           106..145
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             175..201
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             181..207
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgggggatct tggtggcgtg
      121 aaactcccgc acctcttcgg ccagcgcctt gtagaagcgc gtatggcttc tagctatcct
      181 tatgacgtgc ctgactatgc cagcctggga ggaccttcta gcggatccat gagctctggt
      241 gctgcttccg gtacaggtcg tggtcgtcca cgtggtggag gtccaggtcc cggggaccct
      301 ccgcctagcg agacacacaa gcttgtggtc gtgggtggtg tcggcgtggg caagagcgcg
      361 ctgaccatcc agttcatcca gtcctacttc gtgtctgact acgaccccag tattgaggac
      421 tcctacacga agatctgcag tgtggatggc atcccagccc ggctggacat cctggacacc
      481 gcgggccagg aagagttcgg ggccatgaga gagcagtaca tgcgtgctgg ccacggcttc
      541 ctgctggtgt tcgccattaa tgaccggcag agtttcaacg aggtgggcaa gctcttcacg
      601 cagattctgc gggtcaagga ccgcgacgac ttccccgttg tgttggtcgg gaacaaggca
      661 gatctggagt cacagcgcca ggtcccccga tcagaagcct ctgccttcgg cgcctcccac
      721 cacgtagcct actttgaggc ctcggccaaa ctgcgtctca acgtggacga ggcttttgag
      781 cagctggtgc gggctgtccg gaaataccag gaacaagagc tcccaccgag ccctcccagt
      841 gcccccagga agaagggcgg gggctgcccc tgcgtcctcc tgtagggatc ctgagaactt
      901 cagggtgagt ttggggaccc ttgattgttc tttctttttc gctattgtaa aattcatgtt
      961 atatggaggg ggcaaagttt tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca
     1021 ccatggaccc tcatgataat tttgtttctt
//
