LOCUS       sequence-117430-        1485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, 
            codon optimized for expression in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Lucas JE, Siegel JB
  TITLE     Quantitative functional characterization of conserved molecular 
            interactions in the active site of mannitol 2-dehydrogenase.
  JOURNAL   Protein Sci. 2015 Jun;24(6):936-45. doi: 10.1002/pro.2669. Epub 2015
            Apr 2.
  PUBMED    25752240
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.2669"; 
            journalName: "Protein Sci"; date: "2015-06"; volume: "24"; issue: 
            "6"; pages: "936-45"
COMMENT     Sequence Label: pfMDH_WT
FEATURES             Location/Qualifiers
     source          1..1485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaaactga acaaacagaa tctgacccag ctggcaccgg aagttaaact gcctgcatat
       61 accctggcag atacccgtca gggtattgca catattggtg ttggtggttt tcatcgtgca
      121 catcaggcat attataccga tgcactgatg aatacaggtg aaggtctgga ttggagcatt
      181 tgcggtgttg gtctgcgtag cgaagatcgt aaagcacgtg atgatctggc aggtcaggat
      241 tacctgttta ccctgtatga actgggcgat accgatgata ccgaagttcg tgttattggt
      301 agcattagcg atatgctgct ggcagaagat agcgcacagg cactgattga taaactggca
      361 agtccggaaa ttcgtattgt tagcctgacc attaccgaag gtggttattg tattgatgat
      421 agcaacggtg aatttatggc acatctgccg cagattcagc acgatctggc acatccgagc
      481 agcccgaaaa ccgtttttgg ttttatttgt gcagcactga cacagcgtcg tgcagcaggt
      541 attccggcat ttaccgttat gagctgtgat aacctgccgc ataatggtgc agttacccgt
      601 aaagccctgc tggcatttgc agccctgcat aatgcagaac tgcatgattg gattaaagcc
      661 catgttagct ttccgaatgc aatggttgat cgtattaccc cgatgaccag caccgcacat
      721 cgtctgcaac tgcatgacga acatggtatt gatgatgcat ggcctgttgt ttgtgaaccg
      781 tttgttcagt gggttctgga agataaattt gttaatggtc gtccggcatg ggaaaaagtt
      841 ggtgttcagt ttaccgatga tgttaccccg tatgaagaaa tgaaaattgg tctgctgaat
      901 ggtagccatc tggcactgac ctatctgggt tttctgaaag gttatcgttt tgtgcatgaa
      961 accatgaacg atccgctgtt tgttgcctat atgcgtgcat atatggatct ggatgtgacc
     1021 ccgaatctgg ctccggttcc gggtattgat ctgaccgatt ataaacagac cctggttgat
     1081 cgttttagca atcaggcaat tgcagatcag ctggaacgtg tttgtagtga tggtagcagc
     1141 aaatttccta aatttaccgt tccgacaatt aatcgtctga ttgcagatgg tcgtgaaacc
     1201 gaacgtgcag cactggttgt tgcagcatgg gcactgtatc tgaaaggcgt tgatgaaaat
     1261 ggtgtgagct ataccattcc tgatccgcgt gcagaatttt gtcagggtct ggtttcagat
     1321 gatgcactga ttagccagcg tctgctggca gttgaagaaa tttttggcac cgcaattccg
     1381 aatagtccgg aatttgttgc cgcatttgaa cgttgttatg gtagcctgcg tgataatggt
     1441 gttaccacca ccctgaaaca tctgctgaaa aaacctgttg gtagc
//
