LOCUS       sequence-117434-       14655 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  UAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in 
            minTol backbone; also with cry:cfp cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14655)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 14655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
FEATURES             Location/Qualifiers
     source          1..14655
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4043..4137
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4206..4230
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4233..4242
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4269..4298
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4308..4337
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4347..4376
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4386..4415
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4425..4454
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4488..4517
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4542..4562
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4563..5990
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             6003..6059
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(6061..6085)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7245..7265)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     polyA_signal    7305..7439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7329..7348)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7383..7402
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    10566..10773
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     10762..10779
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(10797..11510,11511..11513,11514..11516))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10842..10863)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     11191..11210
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11450..11471
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(12467..12487)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12467..12485)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12506..12522)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12506..12522)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12519..12541)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12530..12546
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12554..12560,12561..12578,12579..12584))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12599..12620
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12737..12754)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12908..13496)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12988..13007)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13667..14458,14459..14527))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14290..14309
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14528..14632)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
      721 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      781 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
      841 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
      901 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
      961 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1021 gatgggccct agggataaca gggtaatact agtggatcga tccgtaatac aattgtacca
     1081 ggttttggtt tattacatgt gactgacggc ttcctgtgcg tgctcaggaa acggcagttg
     1141 ggcactgcac tgcccggtga tggtgccacg gtggctcctg ccgccttctt tgatattcac
     1201 tctgttgtat ttcatctctt cttgccgatg aaaggatata acagtctgtg aggaaatact
     1261 tggtatttct tctgatcagc gtttttataa gtaatgttga atattggata aggctgtgtg
     1321 tcctttgtct tgggagacaa agcccacagc aggtggtggt tggggtggtg gcagctcagt
     1381 gacaggagag gtttttttgc ctgttttttt tgttgttttt tttttttaag taaggtgttc
     1441 ttttttctta gtaaaatttc tactggactg tatgttttga caggtcagaa acatttcttc
     1501 aaaagaagaa ccttttggaa actgtacagc ccttttcttt cattcccttt ttgctttctg
     1561 tgccaatgcc tttggttctg attgcattat ggaaaacgtt gatcggaact tgaggttttt
     1621 atttatagtg tggcttgaaa gcttggatag ctgttgttac atgagatacc ttattaagtt
     1681 taggccagct tgatgcttta tttttttccc tttgaagtag tgagcgttct ctggtttttt
     1741 tcctttgaaa ctggcgaggc ttagattttt ctaatgggat tttttacctg atgatctagt
     1801 tgcataccca aatgcttgta aatgttttcc tagttaacat gttgataact tcggatttac
     1861 atgttgtata tacttgtcat ctgtgtttct agtaaaaata tatggcattt atagaaatac
     1921 gtaattcctg atttcctttt ttttttatct ctatgctctg tgtgtacagg tcaaacagac
     1981 ttcactccta tttttattta tagaatttta tatgcagtct gtcgttggtt cttgtgttgt
     2041 aaggatacag ccttaaattt cctagagcga tgctcagtaa ggcgggttgt cacatgggtt
     2101 caaatgtaaa acgggcacgt ttggctgctg ccttcccgag atccaggaca ctaaactgct
     2161 tctgcactga ggtataaatc gcttcagatc ccagggaagt gtagatccac gtgcatattc
     2221 ttaaagaaga atgaatactt tctaaaatat tttggcatag gaagcaagct gcatggattt
     2281 gtttgggact taaattattt tggtaacgga gtgcataggt tttaaacaca gttgcagcat
     2341 gctaacgagt cacagcattt atgcagaagt gatgcctgtt gcagctgttt acggcactgc
     2401 cttgcagtga gcattgcaga taggggtggg gtgctttgtg tcgtgttccc acacgctgcc
     2461 acacagccac ctcccggaac acatctcacc tgctgggtac ttttcaaacc atcttagcag
     2521 tagtagatga gttactatga aacagagaag ttcctcagtt ggatattctc atgggatgtc
     2581 ttttttccca tgttgggcaa agtatgataa agcatctcta tttgtaaatt atgcacttgt
     2641 tagttcctga atcctttcta tagcaccact tattgcagca ggtgtaggct ctggtgtggc
     2701 ctgtgtctgt gcttcaatct tttaaagctt ctttggaaat acactgactt gattgaagtc
     2761 tcttgaagat agtaaacagt acttaccttt gatcccaatg aaatcgagca tttcagttgt
     2821 aaaagaattc cgcctattca taccatgtaa tgtaatttta cacccccagt gctgacactt
     2881 tggaatatat tcaagtaata gactttggcc tcaccctctt gtgtactgta ttttgtaata
     2941 gaaaatattt taaactgtgc atatgattat ttcattatga aagagacatt ctgctgatct
     3001 tcaaatgtaa gaaaatgagg agtgcgtgtg cttttataaa tacaagtgat tgcaaattag
     3061 tgcaggtgtc cttaaaaaaa aaaaaaagta atataaaaag gaccaggtgt tttacaagtg
     3121 aaatacattc ctatttggta aacagttaca tttttatgaa gattaccagc gctgctgact
     3181 ttctaaacat aaggctgtat tgtcttcctg taccattgca tttcctcatt cccaatttgc
     3241 acaaggatgt ctgggtaaac tattcaagaa atggctttga aatacagcat gggagcttgt
     3301 ctgagttgga atgcagagtt gcactgcaaa atgtcaggaa atggatgtct ctcagaatgc
     3361 ccaactccaa aggattttat atgtgtatat agtaagcagt ttcctgattc cagcaggcca
     3421 aagagtctgc tgaatgttgc gttgccggag acctgtattt ctcaacaagg taagatggta
     3481 tcctagcaac tgcggatttt aatacatttt cagcagaagt acttagttaa tctctacctt
     3541 tagggatcgt ttcatcattt ttagatgtta tacttgaaat actgcataac ttttagcttt
     3601 catgggttcc tttttttcag cctttaggag actgttaagc aatttgctgt ccaacttttg
     3661 tgttggtctt aaactgcaat agtagtttac cttgtattga agaaataaag accattttta
     3721 tattaaaaaa tacttttgtc tgtcttcatt ttgacttgtc tgatatcctt gcagtgctca
     3781 ttatgtcant ctgtcagata tcagacatca aaacttaacg tgagctcagt ggagttacag
     3841 ctgcggtttt gatgctgtta ttatttctga aactagaaat gatgttgtct tcatctgctc
     3901 atcaaacact tcatgcagag tttaaggcta gtgagaaatg catacattta ttgatacttt
     3961 tttaaagtca actttttatc agattttttt ttcatttgga aatatattgt tttctagtgg
     4021 atcagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
     4081 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     4141 agactctaga gggtatataa tggatctcgc gtctcagcct cactttgagc tcctccacac
     4201 gctagccaag tttgtacaaa aaagcaggct ccgccaccat ggctggatcc catcgattta
     4261 aagctatgga gcaaaagctc atttctgaag aggacttgaa tgaaatggag caaaagctca
     4321 tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag gacttgaatg
     4381 aaatggagca aaagctcatt tctgaagagg acttgaatga aatggagcaa aagctcattt
     4441 ctgaagagga cttgaatgaa atggagagct tgggcgacct caccatggag caaaagctca
     4501 tttctgaaga ggacttgaat ttagagatct cgaattccat gcccaagaag aagaggaagg
     4561 tgatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag gtgcgcatgg
     4621 agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg
     4681 agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg
     4741 acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac cccgccgaca
     4801 tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact
     4861 tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac ggcacgctga
     4921 tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta atgcagaaga
     4981 agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc gtgctgaagg
     5041 gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg gagttcaaga
     5101 ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg gacaccaagc
     5161 tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag cgctccgagg
     5221 gccgccacca cctgttcctg gggcatggca ccggcagcac cggcagcggc agctccggca
     5281 ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc ttcaaggtgc
     5341 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     5401 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     5461 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     5521 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     5581 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     5641 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     5701 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     5761 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     5821 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     5881 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     5941 ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag ggaggatccg
     6001 gagccacgaa cttctctctg ttaaagcaag caggagacgt ggaagaaaac cccggtcctc
     6061 acccagcttt cttgtacaaa gtgggaggat caggaatgct cgataagttc aaacccgtgc
     6121 agatcgattc ggtcatggcc ataagcaaga cggtagagtg gctgaaggag cagctagaaa
     6181 cgcgcagaga ctgtttgctc gttatggact gccgagcgca agagctgtac gagtcgtcgc
     6241 acgtcgaaac ggccattaac gtggccatcc cgagcctcat gctccggcga ctcaagaaag
     6301 gcaacctgcc catcaagtct ctgctttcta acggggaaga cagggaaagg ttcgcgcgga
     6361 gatgcaagac ggacacgatc gtgttgtacg acgagtacag ccgcgagtgg aatgaaaaca
     6421 tcgacggcgg ctccgtgttg ggtttactgc tgaggagaat gaaggacgag ggctacaagg
     6481 ctttctatct cgagggtggc ttcagcaaat ttcaaactga ttttcccgca atgtgtgaga
     6541 cgaacctcga cggttcgtca agcagcagtg caccaacctc ccaggtcctg ggtctcggag
     6601 ggctcagaat cagctctgac tcctcggaca tcgagtcgga tatcgaccga gacccaagca
     6661 gtgcaactga ctcagatggc gcaccccttt ccaaccccca gccctcattc cccgtggaga
     6721 tccttccaca tctgtatctg ggctgtgcta aggactccac aaacctggat atcctggagg
     6781 agtttggcat caagtacatc ttgaacgtga cccctaatct ccccaacatg ttcgagaatg
     6841 ccggggagtt taagtacaag cagattccca tctctgatca ctggagccag aacctctcac
     6901 agtttttccc tgaagccatc agctttattg atgaggcccg tggactgaag tgtggcgtgc
     6961 ttgttcactg ccttgcaggc atcagtcgtt ctgtcactgt aacagtggcg tacctcatgc
     7021 agaagctcaa cctgtccatg aacgatgctt atgacattgt caaaatgaag aagtcgaaca
     7081 tctcgcccaa ctttaacttc atgggtcaac tcttggactt tgagcgcacg ttaggactca
     7141 agagtccgtg tgataaccgt gtgccggccc caacccagcc actgtacttc accacaccaa
     7201 ccaatcacaa cgtttttcag ttggaccctc tcgagtccac gtaacaactt tattatacat
     7261 agttgcactc gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa
     7321 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     7381 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     7441 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt
     7501 ggcgcgccga tcccccgacc tcgagggggg gcccggatcg atccgtaata caattgtacc
     7561 aggttttggt ttattacatg tgactgacgg cttcctgtgc gtgctcagga aacggcagtt
     7621 gggcactgca ctgcccggtg atggtgccac ggtggctcct gccgccttct ttgatattca
     7681 ctctgttgta tttcatctct tcttgccgat gaaaggatat aacagtctgt gaggaaatac
     7741 ttggtatttc ttctgatcag cgtttttata agtaatgttg aatattggat aaggctgtgt
     7801 gtcctttgtc ttgggagaca aagcccacag caggtggtgg ttggggtggt ggcagctcag
     7861 tgacaggaga ggtttttttg cctgtttttt ttgttgtttt ttttttttaa gtaaggtgtt
     7921 cttttttctt agtaaaattt ctactggact gtatgttttg acaggtcaga aacatttctt
     7981 caaaagaaga accttttgga aactgtacag cccttttctt tcattccctt tttgctttct
     8041 gtgccaatgc ctttggttct gattgcatta tggaaaacgt tgatcggaac ttgaggtttt
     8101 tatttatagt gtggcttgaa agcttggata gctgttgtta catgagatac cttattaagt
     8161 ttaggccagc ttgatgcttt atttttttcc ctttgaagta gtgagcgttc tctggttttt
     8221 ttcctttgaa actggcgagg cttagatttt tctaatggga ttttttacct gatgatctag
     8281 ttgcataccc aaatgcttgt aaatgttttc ctagttaaca tgttgataac ttcggattta
     8341 catgttgtat atacttgtca tctgtgtttc tagtaaaaat atatggcatt tatagaaata
     8401 cgtaattcct gatttccttt tttttttatc tctatgctct gtgtgtacag gtcaaacaga
     8461 cttcactcct atttttattt atagaatttt atatgcagtc tgtcgttggt tcttgtgttg
     8521 taaggataca gccttaaatt tcctagagcg atgctcagta aggcgggttg tcacatgggt
     8581 tcaaatgtaa aacgggcacg tttggctgct gccttcccga gatccaggac actaaactgc
     8641 ttctgcactg aggtataaat cgcttcagat cccagggaag tgtagatcca cgtgcatatt
     8701 cttaaagaag aatgaatact ttctaaaata ttttggcata ggaagcaagc tgcatggatt
     8761 tgtttgggac ttaaattatt ttggtaacgg agtgcatagg ttttaaacac agttgcagca
     8821 tgctaacgag tcacagcatt tatgcagaag tgatgcctgt tgcagctgtt tacggcactg
     8881 ccttgcagtg agcattgcag ataggggtgg ggtgctttgt gtcgtgttcc cacacgctgc
     8941 cacacagcca cctcccggaa cacatctcac ctgctgggta cttttcaaac catcttagca
     9001 gtagtagatg agttactatg aaacagagaa gttcctcagt tggatattct catgggatgt
     9061 cttttttccc atgttgggca aagtatgata aagcatctct atttgtaaat tatgcacttg
     9121 ttagttcctg aatcctttct atagcaccac ttattgcagc aggtgtaggc tctggtgtgg
     9181 cctgtgtctg tgcttcaatc ttttaaagct tctttggaaa tacactgact tgattgaagt
     9241 ctcttgaaga tagtaaacag tacttacctt tgatcccaat gaaatcgagc atttcagttg
     9301 taaaagaatt ccgcctattc ataccatgta atgtaatttt acacccccag tgctgacact
     9361 ttggaatata ttcaagtaat agactttggc ctcaccctct tgtgtactgt attttgtaat
     9421 agaaaatatt ttaaactgtg catatgatta tttcattatg aaagagacat tctgctgatc
     9481 ttcaaatgta agaaaatgag gagtgcgtgt gcttttataa atacaagtga ttgcaaatta
     9541 gtgcaggtgt ccttaaaaaa aaaaaaaagt aatataaaaa ggaccaggtg ttttacaagt
     9601 gaaatacatt cctatttggt aaacagttac atttttatga agattaccag cgctgctgac
     9661 tttctaaaca taaggctgta ttgtcttcct gtaccattgc atttcctcat tcccaatttg
     9721 cacaaggatg tctgggtaaa ctattcaaga aatggctttg aaatacagca tgggagcttg
     9781 tctgagttgg aatgcagagt tgcactgcaa aatgtcagga aatggatgtc tctcagaatg
     9841 cccaactcca aaggatttta tatgtgtata tagtaagcag tttcctgatt ccagcaggcc
     9901 aaagagtctg ctgaatgttg cgttgccgga gacctgtatt tctcaacaag gtaagatggt
     9961 atcctagcaa ctgcggattt taatacattt tcagcagaag tacttagtta atctctacct
    10021 ttagggatcg tttcatcatt tttagatgtt atacttgaaa tactgcataa cttttagctt
    10081 tcatgggttc ctttttttca gcctttagga gactgttaag caatttgctg tccaactttt
    10141 gtgttggtct taaactgcaa tagtagttta ccttgtattg aagaaataaa gaccattttt
    10201 atattaaaaa atacttttgt ctgtcttcat tttgacttgt ctgatatcct tgcagtgctc
    10261 attatgtcan tctgtcagat atcagacatc aaaacttaac gtgagctcag tggagttaca
    10321 gctgcggttt tgatgctgtt attatttctg aaactagaaa tgatgttgtc ttcatctgct
    10381 catcaaacac ttcatgcaga gtttaaggct agtgagaaat gcatacattt attgatactt
    10441 ttttaaagtc aactttttat cagatttttt tttcatttgg aaatatattg ttttctagag
    10501 tcgacctgca ggaattcgat atcaagctta tcgataccgt cgaccaccgc ggtggcggcg
    10561 atctctcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg tcctgcccca
    10621 ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt tcctcatttt
    10681 attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc
    10741 aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg gccgctttac
    10801 ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc
    10861 atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct caggtagtgg
    10921 ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg
    10981 agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttggcctt gatgccgttc
    11041 ttctgcttgt cggcggtgat atagacgttg tcgctgatgg cgttgtactc cagcttgtgc
    11101 cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg
    11161 gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag
    11221 atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc
    11281 atgtggtcgg ggtagcgggc gaagcactgc acgccccagg tcagggtggt cacgagggtg
    11341 ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag
    11401 gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc
    11461 agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct caccatgttg
    11521 tcagacctgg taactcctta ctgtaactta ttgcacctga tgaacaaaag atgtgaactg
    11581 aacgcaagta ggaggctaat agaggctagt acagtacaaa gatccccaaa tgatgtgtgc
    11641 cggccttagt ggaggcagac ccctggttat atacctgtgc tgcagaaaga ggggcttaca
    11701 aatggatctc tctggaatgg cattatctca tggtgggaac agtgttgtca gcagaatcag
    11761 cggggtggac tctgacctga gaatgacagt ctagcactga atggctcaga caatactctt
    11821 gtggccaact atcaccggaa aataagtgcc tgccaaaaca aaccagacca gatgaagcta
    11881 catggctgta gaacgtcagt atttaatgtg ttgtggccac ttggttcacc agatttaaga
    11941 gctaattttt ttaaatatca cagataatca atcgcttttt ttttttcttg actgaaaagc
    12001 ttagactgga aaattctttg aaaaagtttg cactacagaa aataaataca taacatcctg
    12061 cactgaatgc acactattaa tagatctatt accctgttat ccctaggctc gaggcgaaga
    12121 tacggccacg ggtgctcttg atcctgtggc tgattttgga ctgtgctgct cgcagctgct
    12181 gatgaatcac atacttcctc cattttcttc cactgattga ctgttataat ttccctaatt
    12241 tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac atgacgtcac ttccaaagga
    12301 ccaatgaaca tgtctgacca atttcatata atgtgaaaac gattttcata ggcagaataa
    12361 ataacattta aattaaactg ggcatcagcg caattcaatt ggtttggtaa tagcaaggga
    12421 aaatagaatg aagtgatctc caaaaaatgg tacccagctt ttgttccctt tagtgagggt
    12481 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    12541 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    12601 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    12661 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    12721 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    12781 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    12841 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    12901 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    12961 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    13021 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    13081 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    13141 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    13201 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    13261 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    13321 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
    13381 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    13441 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    13501 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    13561 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    13621 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    13681 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    13741 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    13801 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    13861 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    13921 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    13981 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    14041 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    14101 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    14161 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    14221 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    14281 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    14341 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    14401 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    14461 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    14521 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    14581 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    14641 cccgaaaagt gccac
//
