LOCUS       sequence-117438-       13294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTol Destination vector with MAR-flanked UAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13294)
  AUTHORS   Shin M, Beane TJ, Quillien A, Male I, Zhu LJ, Lawson ND
  TITLE     Vegfa signals through ERK to promote angiogenesis, but not artery 
            differentiation.
  JOURNAL   Development. 2016 Oct 15;143(20):3796-3805. Epub 2016 Aug 30.
  PUBMED    27578780
REFERENCE   2  (bases 1 to 13294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2016-10-15"; volume: "143(20)"; pages: 
            "3796-3805. Epub 2016 Aug 30"
COMMENT     Sequence Label: pmTol-ins-UAS-R1R3-CC
FEATURES             Location/Qualifiers
     source          1..13294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4043..4137
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    4207..4330
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(4355..4360,4361..4378,4379..4385)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             4439..5098
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(4505..4524)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             5440..5745
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     5624..5643
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(5782..5905)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    5945..6079
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5969..5988)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6023..6042
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     polyA_signal    9206..9413
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     9402..9419
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(9437..10150,10151..10153,10154..10156))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9482..9503)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9831..9850
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10090..10111
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(11106..11126)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11106..11124)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11145..11161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11145..11161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11158..11180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11169..11185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11193..11199,11200..11217,11218..11223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11238..11259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11376..11393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11547..12135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11627..11646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12306..13097,13098..13166))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12929..12948
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13167..13271)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca
      661 ccgcggtggc ggccgctctt gtttaagctt ttaatctcaa aaaacattaa atgaaatgca
      721 tacaaggttt tatcctgctt tagaactgtt tgtatttaat tatcaaacta taagacagac
      781 aatctaatgc cagtacacgc tactcaaagt tgtaaaacct cagatttaac ttcagtagaa
      841 gctgattctc aaaattgtta gtgtcaagcc tagctctttt ggggctgaaa agcaatcctg
      901 cagtgctgaa aagcctctca caggcagccg atgcgggaag aggtgtatta gtcttgatag
      961 agaggctgca aatagcagga aacgtgagca gagactccct ggtgtctgaa acacaggcca
     1021 gatgggccct agggataaca gggtaatact agtggatcga tccgtaatac aattgtacca
     1081 ggttttggtt tattacatgt gactgacggc ttcctgtgcg tgctcaggaa acggcagttg
     1141 ggcactgcac tgcccggtga tggtgccacg gtggctcctg ccgccttctt tgatattcac
     1201 tctgttgtat ttcatctctt cttgccgatg aaaggatata acagtctgtg aggaaatact
     1261 tggtatttct tctgatcagc gtttttataa gtaatgttga atattggata aggctgtgtg
     1321 tcctttgtct tgggagacaa agcccacagc aggtggtggt tggggtggtg gcagctcagt
     1381 gacaggagag gtttttttgc ctgttttttt tgttgttttt tttttttaag taaggtgttc
     1441 ttttttctta gtaaaatttc tactggactg tatgttttga caggtcagaa acatttcttc
     1501 aaaagaagaa ccttttggaa actgtacagc ccttttcttt cattcccttt ttgctttctg
     1561 tgccaatgcc tttggttctg attgcattat ggaaaacgtt gatcggaact tgaggttttt
     1621 atttatagtg tggcttgaaa gcttggatag ctgttgttac atgagatacc ttattaagtt
     1681 taggccagct tgatgcttta tttttttccc tttgaagtag tgagcgttct ctggtttttt
     1741 tcctttgaaa ctggcgaggc ttagattttt ctaatgggat tttttacctg atgatctagt
     1801 tgcataccca aatgcttgta aatgttttcc tagttaacat gttgataact tcggatttac
     1861 atgttgtata tacttgtcat ctgtgtttct agtaaaaata tatggcattt atagaaatac
     1921 gtaattcctg atttcctttt ttttttatct ctatgctctg tgtgtacagg tcaaacagac
     1981 ttcactccta tttttattta tagaatttta tatgcagtct gtcgttggtt cttgtgttgt
     2041 aaggatacag ccttaaattt cctagagcga tgctcagtaa ggcgggttgt cacatgggtt
     2101 caaatgtaaa acgggcacgt ttggctgctg ccttcccgag atccaggaca ctaaactgct
     2161 tctgcactga ggtataaatc gcttcagatc ccagggaagt gtagatccac gtgcatattc
     2221 ttaaagaaga atgaatactt tctaaaatat tttggcatag gaagcaagct gcatggattt
     2281 gtttgggact taaattattt tggtaacgga gtgcataggt tttaaacaca gttgcagcat
     2341 gctaacgagt cacagcattt atgcagaagt gatgcctgtt gcagctgttt acggcactgc
     2401 cttgcagtga gcattgcaga taggggtggg gtgctttgtg tcgtgttccc acacgctgcc
     2461 acacagccac ctcccggaac acatctcacc tgctgggtac ttttcaaacc atcttagcag
     2521 tagtagatga gttactatga aacagagaag ttcctcagtt ggatattctc atgggatgtc
     2581 ttttttccca tgttgggcaa agtatgataa agcatctcta tttgtaaatt atgcacttgt
     2641 tagttcctga atcctttcta tagcaccact tattgcagca ggtgtaggct ctggtgtggc
     2701 ctgtgtctgt gcttcaatct tttaaagctt ctttggaaat acactgactt gattgaagtc
     2761 tcttgaagat agtaaacagt acttaccttt gatcccaatg aaatcgagca tttcagttgt
     2821 aaaagaattc cgcctattca taccatgtaa tgtaatttta cacccccagt gctgacactt
     2881 tggaatatat tcaagtaata gactttggcc tcaccctctt gtgtactgta ttttgtaata
     2941 gaaaatattt taaactgtgc atatgattat ttcattatga aagagacatt ctgctgatct
     3001 tcaaatgtaa gaaaatgagg agtgcgtgtg cttttataaa tacaagtgat tgcaaattag
     3061 tgcaggtgtc cttaaaaaaa aaaaaaagta atataaaaag gaccaggtgt tttacaagtg
     3121 aaatacattc ctatttggta aacagttaca tttttatgaa gattaccagc gctgctgact
     3181 ttctaaacat aaggctgtat tgtcttcctg taccattgca tttcctcatt cccaatttgc
     3241 acaaggatgt ctgggtaaac tattcaagaa atggctttga aatacagcat gggagcttgt
     3301 ctgagttgga atgcagagtt gcactgcaaa atgtcaggaa atggatgtct ctcagaatgc
     3361 ccaactccaa aggattttat atgtgtatat agtaagcagt ttcctgattc cagcaggcca
     3421 aagagtctgc tgaatgttgc gttgccggag acctgtattt ctcaacaagg taagatggta
     3481 tcctagcaac tgcggatttt aatacatttt cagcagaagt acttagttaa tctctacctt
     3541 tagggatcgt ttcatcattt ttagatgtta tacttgaaat actgcataac ttttagcttt
     3601 catgggttcc tttttttcag cctttaggag actgttaagc aatttgctgt ccaacttttg
     3661 tgttggtctt aaactgcaat agtagtttac cttgtattga agaaataaag accattttta
     3721 tattaaaaaa tacttttgtc tgtcttcatt ttgacttgtc tgatatcctt gcagtgctca
     3781 ttatgtcant ctgtcagata tcagacatca aaacttaacg tgagctcagt ggagttacag
     3841 ctgcggtttt gatgctgtta ttatttctga aactagaaat gatgttgtct tcatctgctc
     3901 atcaaacact tcatgcagag tttaaggcta gtgagaaatg catacattta ttgatacttt
     3961 tttaaagtca actttttatc agattttttt ttcatttgga aatatattgt tttctagtgg
     4021 atcagcttgc atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag
     4081 cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg
     4141 agactctaga gggtatataa tggatctcgc gtctcagcct cactttgagc tcctccacac
     4201 gctagccaag tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata
     4261 tattaaatta gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc
     4321 agtcatattg gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     4381 taatgtgtgg attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat
     4441 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     4501 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     4561 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     4621 cattcttgcc cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga
     4681 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     4741 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     4801 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     4861 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     4921 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     4981 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     5041 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaaag
     5101 atctggatcc ggcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg
     5161 cggtataaga atatatactg atatgtatac ccgaagtatg tcaaaaagag gtatgctatg
     5221 aagcagcgta ttacagtgac agttgacagc gacagctatc agttgctcaa ggcatatatg
     5281 atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa tgaagcccgt cgtctgcgtg
     5341 ccgaacgctg gaaagcggaa aatcaggaag ggatggctga ggtcgcccgg tttattgaaa
     5401 tgaacggctc ttttgctgac gagaacaggg gctggtgaaa tgcagtttaa ggtttacacc
     5461 tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat tattgacacg
     5521 cccgggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga taaagtctcc
     5581 cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat gaccaccgat
     5641 atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag ccaccgcgaa
     5701 aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc aggctccctt
     5761 atacacagcc agtctgcagt ccatagtgac tggatatgtt gtgttttaca gtattatgta
     5821 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
     5881 cgttcaactt tattatacat agttgcactc gagcctctag aactatagtg agtcgtatta
     5941 cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     6001 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     6061 aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg
     6121 ggaggtgtgg gaggtttttt ggcgcgccga tcccccgacc tcgagggggg gcccggatcg
     6181 atccgtaata caattgtacc aggttttggt ttattacatg tgactgacgg cttcctgtgc
     6241 gtgctcagga aacggcagtt gggcactgca ctgcccggtg atggtgccac ggtggctcct
     6301 gccgccttct ttgatattca ctctgttgta tttcatctct tcttgccgat gaaaggatat
     6361 aacagtctgt gaggaaatac ttggtatttc ttctgatcag cgtttttata agtaatgttg
     6421 aatattggat aaggctgtgt gtcctttgtc ttgggagaca aagcccacag caggtggtgg
     6481 ttggggtggt ggcagctcag tgacaggaga ggtttttttg cctgtttttt ttgttgtttt
     6541 ttttttttaa gtaaggtgtt cttttttctt agtaaaattt ctactggact gtatgttttg
     6601 acaggtcaga aacatttctt caaaagaaga accttttgga aactgtacag cccttttctt
     6661 tcattccctt tttgctttct gtgccaatgc ctttggttct gattgcatta tggaaaacgt
     6721 tgatcggaac ttgaggtttt tatttatagt gtggcttgaa agcttggata gctgttgtta
     6781 catgagatac cttattaagt ttaggccagc ttgatgcttt atttttttcc ctttgaagta
     6841 gtgagcgttc tctggttttt ttcctttgaa actggcgagg cttagatttt tctaatggga
     6901 ttttttacct gatgatctag ttgcataccc aaatgcttgt aaatgttttc ctagttaaca
     6961 tgttgataac ttcggattta catgttgtat atacttgtca tctgtgtttc tagtaaaaat
     7021 atatggcatt tatagaaata cgtaattcct gatttccttt tttttttatc tctatgctct
     7081 gtgtgtacag gtcaaacaga cttcactcct atttttattt atagaatttt atatgcagtc
     7141 tgtcgttggt tcttgtgttg taaggataca gccttaaatt tcctagagcg atgctcagta
     7201 aggcgggttg tcacatgggt tcaaatgtaa aacgggcacg tttggctgct gccttcccga
     7261 gatccaggac actaaactgc ttctgcactg aggtataaat cgcttcagat cccagggaag
     7321 tgtagatcca cgtgcatatt cttaaagaag aatgaatact ttctaaaata ttttggcata
     7381 ggaagcaagc tgcatggatt tgtttgggac ttaaattatt ttggtaacgg agtgcatagg
     7441 ttttaaacac agttgcagca tgctaacgag tcacagcatt tatgcagaag tgatgcctgt
     7501 tgcagctgtt tacggcactg ccttgcagtg agcattgcag ataggggtgg ggtgctttgt
     7561 gtcgtgttcc cacacgctgc cacacagcca cctcccggaa cacatctcac ctgctgggta
     7621 cttttcaaac catcttagca gtagtagatg agttactatg aaacagagaa gttcctcagt
     7681 tggatattct catgggatgt cttttttccc atgttgggca aagtatgata aagcatctct
     7741 atttgtaaat tatgcacttg ttagttcctg aatcctttct atagcaccac ttattgcagc
     7801 aggtgtaggc tctggtgtgg cctgtgtctg tgcttcaatc ttttaaagct tctttggaaa
     7861 tacactgact tgattgaagt ctcttgaaga tagtaaacag tacttacctt tgatcccaat
     7921 gaaatcgagc atttcagttg taaaagaatt ccgcctattc ataccatgta atgtaatttt
     7981 acacccccag tgctgacact ttggaatata ttcaagtaat agactttggc ctcaccctct
     8041 tgtgtactgt attttgtaat agaaaatatt ttaaactgtg catatgatta tttcattatg
     8101 aaagagacat tctgctgatc ttcaaatgta agaaaatgag gagtgcgtgt gcttttataa
     8161 atacaagtga ttgcaaatta gtgcaggtgt ccttaaaaaa aaaaaaaagt aatataaaaa
     8221 ggaccaggtg ttttacaagt gaaatacatt cctatttggt aaacagttac atttttatga
     8281 agattaccag cgctgctgac tttctaaaca taaggctgta ttgtcttcct gtaccattgc
     8341 atttcctcat tcccaatttg cacaaggatg tctgggtaaa ctattcaaga aatggctttg
     8401 aaatacagca tgggagcttg tctgagttgg aatgcagagt tgcactgcaa aatgtcagga
     8461 aatggatgtc tctcagaatg cccaactcca aaggatttta tatgtgtata tagtaagcag
     8521 tttcctgatt ccagcaggcc aaagagtctg ctgaatgttg cgttgccgga gacctgtatt
     8581 tctcaacaag gtaagatggt atcctagcaa ctgcggattt taatacattt tcagcagaag
     8641 tacttagtta atctctacct ttagggatcg tttcatcatt tttagatgtt atacttgaaa
     8701 tactgcataa cttttagctt tcatgggttc ctttttttca gcctttagga gactgttaag
     8761 caatttgctg tccaactttt gtgttggtct taaactgcaa tagtagttta ccttgtattg
     8821 aagaaataaa gaccattttt atattaaaaa atacttttgt ctgtcttcat tttgacttgt
     8881 ctgatatcct tgcagtgctc attatgtcan tctgtcagat atcagacatc aaaacttaac
     8941 gtgagctcag tggagttaca gctgcggttt tgatgctgtt attatttctg aaactagaaa
     9001 tgatgttgtc ttcatctgct catcaaacac ttcatgcaga gtttaaggct agtgagaaat
     9061 gcatacattt attgatactt ttttaaagtc aactttttat cagatttttt tttcatttgg
     9121 aaatatattg ttttctagag tcgacctgca ggaattcgat atcaagctta tcgataccgt
     9181 cgaccaccgc ggtggcggcg atctctcccc agcatgcctg ctattgtctt cccaatcctc
     9241 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     9301 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     9361 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc
     9421 tgatcagcgg gccgctttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     9481 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg
     9541 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
     9601 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
     9661 agttggcctt gatgccgttc ttctgcttgt cggcggtgat atagacgttg tcgctgatgg
     9721 cgttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
     9781 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
     9841 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
     9901 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc gaagcactgc acgccccagg
     9961 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
    10021 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
    10081 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
    10141 cgcccttgct caccatgttg tcagacctgg taactcctta ctgtaactta ttgcacctga
    10201 tgaacaaaag atgtgaactg aacgcaagta ggaggctaat agaggctagt acagtacaaa
    10261 gatccccaaa tgatgtgtgc cggccttagt ggaggcagac ccctggttat atacctgtgc
    10321 tgcagaaaga ggggcttaca aatggatctc tctggaatgg cattatctca tggtgggaac
    10381 agtgttgtca gcagaatcag cggggtggac tctgacctga gaatgacagt ctagcactga
    10441 atggctcaga caatactctt gtggccaact atcaccggaa aataagtgcc tgccaaaaca
    10501 aaccagacca gatgaagcta catggctgta gaacgtcagt atttaatgtg ttgtggccac
    10561 ttggttcacc agatttaaga gctaattttt ttaaatatca cagataatca atcgcttttt
    10621 ttttttcttg actgaaaagc ttagactgga aaattctttg aaaaagtttg cactacagaa
    10681 aataaataca taacatcctg cactgaatgc acactattaa tagatctatt accctgttat
    10741 ccctaggctc gaggcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
    10801 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
    10861 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
    10921 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
    10981 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
    11041 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaatgg taccagcttt
    11101 tgttcccttt agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct
    11161 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
    11221 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
    11281 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
    11341 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
    11401 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
    11461 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
    11521 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
    11581 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
    11641 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
    11701 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
    11761 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
    11821 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
    11881 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
    11941 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
    12001 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
    12061 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
    12121 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
    12181 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
    12241 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
    12301 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
    12361 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
    12421 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
    12481 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
    12541 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
    12601 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
    12661 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
    12721 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
    12781 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
    12841 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
    12901 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
    12961 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
    13021 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
    13081 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
    13141 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
    13201 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
    13261 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
