LOCUS       sequence-117444-        3167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Pr promoter expressing a deGFP that is missing a ribosomal binding 
            site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr-delRBS-deGFP
FEATURES             Location/Qualifiers
     source          1..3167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(158..179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(419..438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     766..787
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(982..999)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1153..1741)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1233..1252)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1912..2703,2704..2772))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2535..2554
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2773..2877)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2945..2963
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgtgtgct gttccgctgg
       61 gcatgctgag ctaacaccgt gcgtgttgac aattttacct ctggcggtga taatggttgc
      121 agctagcatg gagcttttca ctggcgttgt tcccatcctg gtcgagctgg acggcgacgt
      181 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      241 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      301 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
      361 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      421 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      481 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      541 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      601 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      661 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      721 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
      781 gttcgtgacc gccgccggga tctaactcga gcaaagcccg ccgaaaggcg ggcttttctg
      841 tgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc
      901 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg
      961 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     1021 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     1081 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     1141 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     1201 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     1261 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     1321 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     1381 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     1441 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     1501 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     1561 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     1621 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1681 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1741 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1801 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1861 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1921 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1981 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     2041 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     2101 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     2161 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     2221 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     2281 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     2341 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     2401 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     2461 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     2521 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     2581 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2641 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2701 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2761 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2821 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2881 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2941 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa ttctggcgaa tcctctgacc
     3001 agccagaaaa cgacctttct gtggtgaaac cggatgctgc aattcagagc ggcagcaagt
     3061 gggggacagc agaagacctg accgccgcag agtggatgtt tgacatggtg aagactatcg
     3121 caccatcagc cagaaaaccg aattttgctg ggtgggctaa cgatatc
//
