LOCUS       sequence-117448-        5176 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  OR2-OR1-Pr1 promoter expressing T7 RNAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Siegal-Gaskins D, Tuza ZA, Kim J, Noireaux V, Murray RM
  TITLE     Gene circuit performance characterization and resource usage in a 
            cell-free ""breadboard"".
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):416-25. doi: 10.1021/sb400203p. 
            Epub 2014 Apr 7.
  PUBMED    24670245
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400203p";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "416-25"
COMMENT     Sequence Label: Pr1-T7RNAP
FEATURES             Location/Qualifiers
     source          1..5176
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..72
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     RBS             410..432
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(3267..3284)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3438..4026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3518..3537)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4197..4988,4989..5057))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4820..4839
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5058..5162)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
       61 gtatcacgag gccctttcgt cttcaagaat tctggcgaat cctctgacca gccagaaaac
      121 gacctttctg tggtgaaacc ggatgctgca attcagagcg gcagcaagtg ggggacagca
      181 gaagacctga ccgccgcaga gtggatgttt gacatggtga agactatcgc accatcagcc
      241 agaaaaccga attttgctgg gtgggctaac gatatccgcc tgatgcgtga acgtgacgga
      301 cgtaaccacc gcgacatgtg tgtgctgttc cgctgggcat gctgagctaa caccgtgcgt
      361 gtagacaatt ttacctctgg cggtgataat ggttgcagct agcaataatt ttgtttaact
      421 ttaagaagga gatataccat gaacacgatt aacatcgcta agaacgactt ctctgacatc
      481 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc
      541 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg
      601 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc
      661 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct
      721 aagcgcggca agcgcccgac agccttccag ttcctgcaag aaatcaagcc ggaagccgta
      781 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt
      841 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc
      901 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta
      961 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt
     1021 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta
     1081 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct
     1141 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc
     1201 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct
     1261 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg
     1321 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct
     1381 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc
     1441 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg
     1501 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc
     1561 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc
     1621 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc
     1681 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg
     1741 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag
     1801 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg
     1861 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag
     1921 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc
     1981 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg
     2041 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta
     2101 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt
     2161 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta
     2221 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag
     2281 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc
     2341 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc
     2401 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct
     2461 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa
     2521 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag
     2581 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct
     2641 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag
     2701 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag
     2761 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact
     2821 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc
     2881 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac
     2941 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac
     3001 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac
     3061 atcttagagt cggacttcgc gttcgcgtaa ctcgagcaaa gcccgccgaa aggcgggctt
     3121 ttctgtgtcg accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc
     3181 ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac
     3241 aggtgccggc agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     3301 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     3361 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     3421 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     3481 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     3541 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     3601 tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta tctcagttcg
     3661 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     3721 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     3781 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     3841 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     3901 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     3961 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     4021 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     4081 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     4141 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     4201 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     4261 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     4321 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     4381 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     4441 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     4501 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     4561 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     4621 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     4681 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     4741 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     4801 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     4861 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     4921 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     4981 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     5041 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     5101 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     5161 cgcacatttc cccgaa
//
