LOCUS       sequence-117469-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of transmembrane and the intracellular domains of Crb 
            with GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  TITLE     Marta Llimargas Casanova plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUASt- Crb intraGFP
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(275..295)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          425..490
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
ORIGIN
        1 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
       61 ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct ctagtcaagg
      121 cactatacat caaatatttt tccataattt tcttgtatag cagtgcagct ttttcctttg
      181 tggtgtaaat agcaaagcaa gcaagagttc tattactaaa cacagcatga ctcaaaaaac
      241 ttagcaattc tgaaggaaag tccttggggt cttctacctt tctcttcttt tttggaggag
      301 tagaatgttg agagtcagca gtagcctcat catcactaga tggcatttct tctgagcaaa
      361 acaggttttc ctcattaaag gcattccacc actgctccca ttcatcagtt ccataggttg
      421 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
      481 ttatatttac cttagagctt taaatctctg taggtagttt gtccaattat gtcacaccac
      541 agaagtaagg ttccttcaca aagatcctct agagcaaaat atgtttttta tttgatttac
      601 ttaaccaata ctttttctgt tctagttgtc attgcttttc gttactatat ttttttttgt
      661 tataaggcca catttactga tatatatact cgcgtatata taggcatata ggattttctg
      721 tttttaattt cgattttagc tcagctaatt tagtttaaga gtcggcaaac aatcttattt
      781 acaaaaatac tcatacaata catttgatgg gcttccttgt ggaatgcaaa ggggaaatcg
      841 acgtcggagc tcaactttgt tcgcagcttt aggattacat atacgagaaa tcgaatactg
      901 aatctaaact atcggaacat aactacataa cgactatagg gcggtacgta tgtcatctaa
      961 actactagaa taaatagaca acggcattat taactaacta aagcgtaggc agggg
//
