LOCUS       sequence-117486-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses two dimeric Broccoli aptamers in the F30 scaffold in 
            bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Filonov GS, Jaffrey SR
  TITLE     RNA Imaging with Dimeric Broccoli in Live Bacterial and Mammalian 
            Cells.
  JOURNAL   Curr Protoc Chem Biol. 2016 Mar 16;8(1):1-28. doi: 
            10.1002/9780470559277.ch150174.
  PUBMED    26995352
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/9780470559277"; journalName: "Curr Protoc Chem Biol"; date:
            "2016-03-16- 16"; volume: "8"; issue: "1"; pages: "1-28"
COMMENT     Sequence Label: pET28c-F30-2xdBroccoli
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 agacgggcaa cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt
       61 ccacgctggt ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat
      121 aacatgagct gtcttcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca
      181 gcccggactc ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca
      241 tcgcagtggg aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg
      301 cactccagtc gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat
      361 gccagccagc cagacgcaga cgcgccgaga cagaacttaa tgggcccgct aacagcgcga
      421 tttgctggtg acccaatgcg accagatgct ccacgcccag tcgcgtaccg tcttcatggg
      481 agaaaataat actgttgatg ggtgtctggt cagagacatc aagaaataac gccggaacat
      541 tagtgcaggc agcttccaca gcaatggcat cctggtcatc cagcggatag ttaatgatca
      601 gcccactgac gcgttgcgcg agaagattgt gcaccgccgc tttacaggct tcgacgccgc
      661 ttcgttctac catcgacacc accacgctgg cacccagttg atcggcgcga gatttaatcg
      721 ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga ggtggcaacg ccaatcagca
      781 acgactgttt gcccgccagt tgttgtgcca cgcggttggg aa
//
