LOCUS       sequence-117487-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses V5- and SBP-tagged RRp41 in mamalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 877)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HsRRP41
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             165..278
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcta gcgctaccgg tcgccaccat gggtaagcct atccctaacc
      121 ctctcctcgg tctcgattct acgcccggaa gcggtagcgg tacgatggac gagaagacca
      181 ccggctgggg gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg
      241 ccaggctgga gcaccaccct cagggccagc gggagcccag atctcgagct caagcttcga
      301 attccatggc ggggctggag ctcttgtcgg accagggcta ccgggtggac gggcggcgcg
      361 ccggggagct gcgcaagatc caggcgcgga tgggcgtgtt cgcgcaggct gacggctcgg
      421 cctacattga gcagggcaac accaaggcac tggctgtggt ctacggcccg cacgagatcc
      481 ggggctcccg ggctcgagcc ctgccggaca gggccctagt gaactgtcaa tatagttcag
      541 cgaccttcag cacaggtgag cgcaagcgac ggccacatgg ggaccgtaag tcctgtgaga
      601 tgggcctgca gctccgccag actttcgaag cagccatcct cacacagctg cacccacgct
      661 cccagattga tatctatgtg caggtgctac aggcagatgg tgggacctat gcagcttgtg
      721 tgaatgcagc cacgctggca gtgctggatg ccgggatacc catgagagac tttgtgtgtg
      781 cgtgctcagc tggcttcgtg gacggcacag ccctggcgga cctcagccat gtggaggaag
      841 cagctggtgg cccccagctg gccctggccc tgctgcc
//
