LOCUS       sequence-117504-         771 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of His-tagged roGFP2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 771)
  TITLE     pQE-60 roGFP2-His
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pQE-60 roGFP2-His
FEATURES             Location/Qualifiers
     source          1..771
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    43..59
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             78..89
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             join(96..98,99..101,102..614)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(141..162)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(402..421)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     749..770
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat
       61 ttcacacaga attcattaaa gaggagaaat taaccatggt gagcaagggc gaggagctgt
      121 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      181 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      241 ccaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      301 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      361 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
      421 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
      481 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaactgcc
      541 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
      601 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
      661 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacctgc tccgccctga
      721 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg a
//
