LOCUS       sequence-117509-         906 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5- and SBP-tagged hnRNPC1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  TITLE     Izaurralde lab hnRNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HshnRNPC1
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..143
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             165..278
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcta gcgctaccgg tcgccaccat gggtaagcct atccctaacc
      121 ctctcctcgg tctcgattct acgcccggaa gcggtagcgg tacgatggac gagaagacca
      181 ccggctgggg gggcggccac gtggtggagg gcctggccgg cgagctggag cagctgcggg
      241 ccaggctgga gcaccaccct cagggccagc gggagcccag atctcgagac atggccagca
      301 acgttaccaa caagacagat cctcgctcca tgaactcccg tgtattcatt gggaatctca
      361 acactcttgt ggtcaagaaa tctgatgtgg aggcaatctt ttcgaagtat ggcaaaattg
      421 tgggctgctc tgttcataag ggctttgcct tcgttcagta tgttaatgag agaaatgccc
      481 gggctgctgt agcaggagag gatggcagaa tgattgctgg ccaggtttta gatattaacc
      541 tggctgcaga gccaaaagtg aaccgaggaa aagcaggtgt gaaacgatct gcagcggaga
      601 tgtacggctc ctcttttgac ttggactatg actttcaacg ggactattat gataggatgt
      661 acagttaccc agcacgtgta cctcctcctc ctcctattgc tcgggctgta gtgccctcga
      721 aacgtcagcg tgtatcagga aacacttcac gaaggggcaa aagtggcttc aattctaaga
      781 gtggacagcg gggatcttcc aagtctggaa agttgaaagg agatgacctt caggccatta
      841 agaaggagct gacccagata aaacaaaaag tggattctct cctggaaaac ctggaaaaaa
      901 ttgaaa
//
