LOCUS       sequence-117511-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  TITLE     Izaurralde lab hnRNP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pT7-V5-SBP-C1-HshnRNPE1
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     48..67
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        48..66
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      94..103
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..144
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             166..279
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWGGGHVVEGLAGELEQLRARLEHHPQGQREP"
ORIGIN
        1 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
       61 tatagggaga cccaagctct agcgctaccg gtcgccacca tgggtaagcc tatccctaac
      121 cctctcctcg gtctcgattc tacgcccgga agcggtagcg gtacgatgga cgagaagacc
      181 accggctggg ggggcggcca cgtggtggag ggcctggccg gcgagctgga gcagctgcgg
      241 gccaggctgg agcaccaccc tcagggccag cgggagccca gatctcgagg gatggatgcc
      301 ggtgtgactg aaagtggact aaatgtgact ctcaccattc ggcttcttat gcacggaaag
      361 gaagtaggaa gcatcattgg gaagaaaggg gagtcggtta agaggatccg cgaggagagt
      421 ggcgcgcgga tcaacatctc ggaggggaat tgtccggaga gaatcatcac tctgaccggc
      481 cccaccaatg ccatctttaa ggctttcgct atgatcatcg acaagctgga ggaagatatc
      541 aacagctcca tgaccaacag taccgcggcc agcaggcccc cggtcaccct gaggctggtg
      601 gtgccggcca cccagtgcgg ctccctgatt gggaaaggcg ggtgtaagat caaagagatc
      661 cgcgagagta cgggggcgca ggtccaggtg gcgggggata tgctgcccaa ctccaccgag
      721 cgggccatca ccatcgctgg cgtgccgcag tctgtcaccg agtgtgtcaa gcagatttgc
      781 ctggtcatgc tggagacgct ctcccagtct ccgcaaggga gagtcatgac cattccgtac
      841 cagcccatgc cggccagctc cccagtcatc tgcgcgggcg gccaagatcg gtgcagcgac
      901 gctgcgggct acccccatgc cacccat
//
