LOCUS       sequence-117527-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses lambdaN-HA-tagged XRN1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Braun JE, Truffault V, Boland A, Huntzinger E, Chang CT, Haas G, 
            Weichenrieder O, Coles M, Izaurralde E
  TITLE     A direct interaction between DCP1 and XRN1 couples mRNA decapping to
            5' exonucleolytic degradation.
  JOURNAL   Nat Struct Mol Biol. 2012 Dec;19(12):1324-31. doi: 
            10.1038/nsmb.2413. Epub 2012 Nov 11.
  PUBMED    23142987
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.2413";
            journalName: "Nat Struct Mol Biol"; date: "2012-12"; volume: "19"; 
            issue: "12"; pages: "1324-31"
COMMENT     Sequence Label: pLNHA-C1-HsXRN1
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             99..164
                     /codon_start=1
                     /product="N-terminal RNA-binding domain from the lambda 
                     bacteriophage antiterminator protein N (Baron-Benhamou et 
                     al., 2004)"
                     /label=lambda N peptide
                     /translation="MDAQTRRRERRAEKQAQWKAAN"
     CDS             189..215
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagctcta gcgctaccgg tcgccaccat ggacgcacaa acacgacgac
      121 gtgagcgtcg cgctgagaaa caagctcaat ggaaagctgc aaacggtgga tctcctccac
      181 atatggctta cccatacgat gttccagatt acgctcctcc atctcgagct caagcttcga
      241 attctgcagt cgacggtacc gcgggcatgg gagtccccaa gttttacaga tggatctcag
      301 agcggtatcc ctgtctcagc gaagtggtga aagagcatca gattcctgaa tttgacaact
      361 tgtacctgga tatgaatgga attatacatc agtgctccca tcctaatgat gatgatgttc
      421 actttagaat ttcagatgat aaaatcttta ctgatatttt tcactacctg gaggtgttgt
      481 ttcgcattat taaacccagg aaagtgttct ttatggctgt agatggtgtg gctcctcgag
      541 caaaaatgaa ccagcagcgt gggaggcgtt ttaggtcagc aaaggaggca gaagacaaaa
      601 ttaaaaaggc aatagagaag ggagaaactc ttcctacaga ggccagattt gattccaact
      661 gtatcacacc aggaactgaa tttatggcca ggttacatga acatctgaag tattttgtaa
      721 atatgaaaat ttccacagac aagtcatggc aaggagttac catctacttc tcaggccatg
      781 agactcctgg agaaggagag cataaaatca tggaatttat cagatccgag aaagcaaagc
      841 cagatcatga tccaaacacc agacactgtc tttatggttt agatgctgac ttgattatgc
      901 ttggattaac aagtcatgag gcacattttt c
//
