LOCUS       sequence-117531-         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Petersen CP, Bordeleau ME, Pelletier J, Sharp PA
  TITLE     Short RNAs Repress Translation after Initiation in Mammalian Cells.
  JOURNAL   Mol Cell. 2006 Feb 17. 21(4):533-42.
  PUBMED    16483934
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2006 Feb 17. 21(4):533-42."
COMMENT     Sequence Label: pFR_HCV_xb
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      61..70
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(133..151)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 acaccgagcg accctgcagc gacccgctta aaagcttggc attccggtac tgttggtaaa
       61 gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
      121 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
      181 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
      241 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
      301 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
      361 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
      421 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
      481 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
      541 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
      601 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
      661 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
      721 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
      781 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgata
//
