LOCUS       sequence-117533-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Tantama M, Hung YP, Yellen G
  TITLE     Imaging Intracellular pH in Live Cells with a Genetically Encoded 
            Red Fluorescent Protein Sensor.
  JOURNAL   J Am Chem Soc. 2011 Jul 6. 133(26):10034-7.
  PUBMED    21631110
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2011 Jul 6. 133(26):10034-7."
COMMENT     Sequence Label: GW1-Mito-pHRed
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(135..797,798..800,801..803))
                     /codon_start=1
                     /product="red fluorescent protein that serves as a 
                     ratiometric pH sensor (Tantama et al., 2011)"
                     /label=pHRed
                     /note="contains an A213S mutation relative to the parental 
                     mKeima protein"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEISIARHSLLG
                     "
ORIGIN
        1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt
       61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc aatgaattcg
      121 tcgactcagc tagctcaacc cagcagagag tggcgggcga tagagatttc acactgctca
      181 acgctggtgt agtcgcggtt gtgagaggta acatccagct tgcggtcgat ttcgtggcgg
      241 ccaggcatgc gaacaggctt tttagccttg taagtgctct tgaactcgca caggtagtga
      301 ccgccgcctt ccagtttcag ggccatataa tcgttaccaa tcagcatacc gtcgcgggca
      361 aacaggcgtt cggtgctggg ctcccagcct tgggttttct tctgcataac tgggccgtta
      421 ggtgggaagt tctcgccgga aatcttaacg ttgtagatga agcagttacc ctggatggag
      481 ctgtcgttgg aaacggtaca aactgcgccg tcctcgaagt tcatagagcg ctcccaggtg
      541 tagccctctg ggaagctctg cttaaagtag tcagggatgt cctcggggta tttggtgaaa
      601 gggatggagc cgtattggag ctgaggggac aggatatccc aggcgaaagg cagtgggccg
      661 cctttggtaa cggtcagctt aacggtctgc tcgccctcgt aaggcttgcc cttgccgtca
      721 ccttcaacct cgaagtagtg gccgttaacg gtgccagaca tataaacttt ataggtcatt
      781 tgctttgcga taacggaaac cataccaccg cttgcgccgc cgcttgcacc gcccatgaga
      841 tagcgccgg
//
