LOCUS       sequence-117544-         886 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 886)
  AUTHORS   Fuerer C, Nusse R
  TITLE     Lentiviral vectors to probe and manipulate the wnt signaling 
            pathway.
  JOURNAL   PLoS One. 2010  . 5(2):e9370.
  PUBMED    20186325
REFERENCE   2  (bases 1 to 886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One. 
            2010  . 5(2):e9370."
COMMENT     Sequence Label: EdTP
FEATURES             Location/Qualifiers
     source          1..886
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     37..56
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        37..55
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             139..162
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact atagggagac
       61 ccaagctggc taggtaagct tggtaccgag ctcggatcca ctagtccagt gtggtggaat
      121 tctgcagata acaccatgga ctacaaagac gatgacgata aaaaggatcc gagctcgaat
      181 tccgaaaact cctcggcaga gagggattta gctgatgtca aatcgtctct agtcaatgaa
      241 tcagaaacga atcaaaacag ctcctccgat tccgaggcgg aaagacggcc tccgcctcgc
      301 tccgaaagtt tccgagacaa atcccgggaa agtttggaag aagcggccaa gaggcaagat
      361 ggagggctct ttaaggggcc accgtatccc ggctacccct tcatcatgat ccccgacctg
      421 acgagcccct acctccccaa cggatcgctc tcgcccaccg cccgaaccta tctccagatg
      481 aaatggccac tgcttgatgt ccaggcaggg agcctccaga gtagacaagc cctcaaggat
      541 gcccggtccc catcaccggc acacattgtc tctaacaaag tgccagtggt gcagcaccct
      601 caccatgtcc accccctcac gcctcttatc acgtacagca atgaacactt cacgccggga
      661 aacccacctc cacacttacc agccgacgta gaccccaaaa caggaatccc acggcctccg
      721 caccctccag atatatcccc gtattaccca ctatcgcctg gcaccgtagg acaaatcccc
      781 catccgctag gatggttagt accacagcaa ggtcaaccag tgtacccaat cacgacagga
      841 ggattcagac acccctaccc cacagctctg accgtcaatg cttcca
//
