LOCUS       sequence-117560-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13P-iCLHN
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..523
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 tggcaaagaa atggtcttca cactcgaaga tttcgttggg gactggcgac agacagccgg
       61 ctacaacctg gaccaagtcc ttgaacaggg aggtgtgtcc agtttgtttc agaatctcgg
      121 ggtgtccgta actccgatcc aaaggattgt cctgagcggt gaaaatgggc tgaagatcga
      181 catccatgtc atcatcccgt atgaaggtct gagcggcgac caaatgggcc agatcgaaaa
      241 aatttttaag gtggtgtacc ctgtggatga tcatcacttt aaggtgatcc tgcactatgg
      301 cacactggta atcgacgggg ttacgccgaa catgatcgac tatttcggac ggccgtatga
      361 aggcatcgcc gtgttcgacg gcaaaaagat cactgtaaca gggaccctgt ggaacggcaa
      421 caaaattatc gacgagcgcc tgatcaaccc cgacggctcc ctgctgttcc gagtaaccat
      481 caacggagtg accggctggc ggctgtgcga acgcattctg gcgggctcga gcggcggatc
      541 cgaaatcggt actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat
      601 gcactacgtc gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc
      661 gacctcctcc tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat
      721 tgctccagac ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga
      781 cgaccacgtc cgcttcatgg atgccttcat cgaa
//
