LOCUS       sequence-117566-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of rat ErbB2 tagged with YFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Lerdrup M, Bruun S, Grandal MV, Roepstorff K, Kristensen MM, 
            Hommelgaard AM, van Deurs B
  TITLE     Endocytic down-regulation of ErbB2 is stimulated by cleavage of its 
            C-terminus.
  JOURNAL   Mol Biol Cell. 2007 Sep;18(9):3656-66. Epub 2007 Jul 11.
  PUBMED    17626164
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Biol 
            Cell."; date: "2007-09"; volume: "18(9)"; pages: "3656-66. Epub 2007
            Jul 11"
COMMENT     Sequence Label: pYFP-ErbB2
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(113..115,116..118,119..631)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(158..179)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(419..438)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gtgaaccgtc agatccgcta gcatgatcat catggagctg gcggcctggt gccgctgggg
       61 gttcctcctc gccctcctgc cccccggaat cgcggtcgac aaagaagtac taatggtgag
      121 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
      181 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
      241 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
      301 caccttcggc tacggcctgc agtgcttcgc ccgctacccc gaccacatga agcagcacga
      361 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
      421 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
      481 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
      541 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
      601 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
      661 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
      721 ctaccagtcc gccctgagc
//
