LOCUS       sequence-117568-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCLHN
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    121..650
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 ccaggaaccc ctgtagggaa ggggcaggag agccaggggc atgagatggt ggacgaggaa
       61 gggggacagg gaagcctgag cgcctctcct gggcttgcca aggactcaaa cccagaagcc
      121 cagagcaggg ccttagggaa gcgggaccct gctctgggcg gaggaatatg tcccagatag
      181 cactggggac tctttaagga aagaaggatg gagaaagaga aagggagtag aggcggccac
      241 gacctggtga acacctagga cgcaccattc tcacaaaggg agttttccac acggacaccc
      301 ccctcctcac cacagccctg ccaggacggg gctggctact ggccttatct cacaggtaaa
      361 actgacgcac ggaggaacaa tataaattgg ggactagaaa ggtgaagagc caaagttaga
      421 actcaggacc aacttattct gattttgttt ttccaaactg cttctcctct tgggaagtgt
      481 aaggaagctg cagcaccagg atcagtgaaa cgcaccagac agccgcgtca gagcagctca
      541 ggttctggga gagggtagcg cagggtggcc actgagaacc gggcaggtca cgcatccccc
      601 ccttccctcc caccccctgc caagctctcc ctcccaggat cctctctggc tccatcgtaa
      661 gcaaacctta gaggttctgg caaggagaga gatggctcca ggaaatgggg gtgtgtcacc
      721 agataaggaa tctgcctaac aggaggtggg ggttagaccc aatatcagga gactaggaag
      781 gaggaggcct aaggatgggg cttttctgtc accaatcctg tccctagtgt ttaaacaata
      841 ttctcactga ctccgtcctg g
//
