LOCUS       sequence-117571-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCLHN
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    202..703
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
ORIGIN
        1 atgattacgc cgcggccgct gctttctctg accagcattc tctcccctgg gcctgtgccg
       61 ctttctgtct gcagcttgtg gcctgggtca cctctacggc tggcccagat ccttccctgc
      121 cgcctccttc aggttccgtc ttcctccact ccctcttccc cttgctctct gctgtgttgc
      181 tgcccaagga tgctctttcc ggagcacttc cttctcggcg ctgcaccacg tgatgtcctc
      241 tgagcggatc ctccccgtgt ctgggtcctc tccgggcatc tctcctccct cacccaaccc
      301 catgccgtct tcactcgctg ggttcccttt tccttctcct tctggggcct gtgccatctc
      361 tcgtttctta ggatggcctt ctccgacgga tgtctccctt gcgtcccgcc tccccttctt
      421 gtaggcctgc atcatcaccg tttttctgga caaccccaaa gtaccccgtc tccctggctt
      481 tagccacctc tccatcctct tgctttcttt gcctggacac cccgttctcc tgtggattcg
      541 ggtcacctct cactcctttc atttgggcag ctcccctacc ccccttacct ctctagtctg
      601 tgctagctct tccagccccc tgtcatggca tcttccaggg gtccgagagc tcagctagtc
      661 ttcttcctcc aacccgggcc cctatgtcca cttcaggaca gcatgtttgc tgcctccagg
      721 gatcctgtgt ccccgagctg ggaccacctt atattcccag ggccggttaa tgtggctctg
      781 gttctgggta cttttatctg tcccctccac cccacagtgg g
//
