LOCUS       sequence-117573-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the G108C/P186C mutant of Sulfolobus solfataricus PCNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA1 G108C/P186C
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(777..794)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(804..821)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             840..862
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    877..901
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(901..920)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(902..920)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatccgc tcttcacagg cgcggcgcaa tccagaagat catgtgaccg
       61 ccgctaactg cttccacatc aattttcatc ggcagatttt caccgaaaga caccatcgtc
      121 ggcgcactaa aaccacgcag gcctttcacc gcatctttga acatttctgc gctgtagcta
      181 ctagaggcag aggtatcgat gctcagttct ttcaggcatt tatctttcat cagaaaggcc
      241 acatagcgtt taccttcttc gcccgcttca attttgattt tatcttcttc cgtgctgata
      301 cgcatttctt caccaaccag ggtcacatcg gccgcgataa cgttcagcac gctttcatcc
      361 gtggtaaaat taacggccag gttcactttc ggttccgtca gctgttcaac ctggcctttt
      421 tctgctttga tgtagatggt acttttggcg caagattttt catcacggat gatgattttc
      481 aggccagaat ccgtttcggt cagttcaatc gtcgcttttt tgctacttgc tttgctcaga
      541 attttcttca cagagctcac atccagtttc acagaggtcg ggctatcaat actgtattca
      601 gacagaacat ctttcggaat gcgcatgatt gccatcagca ctttatcttc cgtcagatga
      661 cgactgaaga tgccatcttc ggtgaagttc aggatgatag aatccgtaac gttggtaatg
      721 ctgttgatga agctaaagaa atctttggcg ttcggataca caattttaaa catatggctg
      781 ccgcgcggca ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat
      841 ctccttctta aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc
      901 ccctatagtg agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc
      961 atcgtggccg gcat
//
