LOCUS       sequence-117574-         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAVS1 safe harbor gene targeting donor expressing CAG-driven 
            Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pAAVS1P-iCLHN
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(40..99)
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc ttgttcaata ggaccggggt tactttcaac
       61 atcgccagcg agtttcaaca aagcgtagtt agtacattga ccggaaatct ccagagtaga
      121 cagccagcgc gcgatctcgc tgccgatcag gtccgggttg tcttcttgca gcagattcag
      181 acccgggccg atgtccacag ccttgcagtt aggcaggctt ttggccaggc gagcggcttc
      241 ggccggtggg atcagaacgc ctggggtgcc ccagaacagc agcttcggga caggggactg
      301 gtgcagccag tccatgtatt cttcgaccag cgcgacgatg ttcgctggct caccggcgat
      361 tggcagctcg tttgggaagc gccacagtgg ctcgcggtca acaggattca ggaacggctc
      421 gcggtaatgg tccatctcga cttcagtcag cgggcggacg acacccatcg gcagcgtacc
      481 ctcgataaaa acgttctgat cgatgatcag cttgcggccg acgtcggtgg tgcggaaggc
      541 ctggaaggtc tcgcgggcaa attctggcca ttcgtcccag gtcgggatag ggcggatgaa
      601 ctccataaat gcaatacctt tgacgcgctc tggattgcgc ttggcccagt ggaaacccag
      661 agcggagccc cagtcgtgaa tgaccaggac gacctcttcc agacccaggg cttcgatgaa
      721 ggcatccatg aagcggacgt ggtcgtcgaa gaaataaccc aggtctggtt tgtcggattt
      781 gcccataccg atcaggtctg gagcaatgca gcgatgggtc ggtgcaacat gcgggatgat
      841 gttgcgccac acgtaggagg aggtcgggtt accgtgcagg aacagcacag gggtgccatc
      901 gcgcggacca acatcgacgt
//
