LOCUS       sequence-117575-         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the wild type of Sulfolobus solfataricus PCNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA2 WT
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(768..785)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(795..812)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             831..853
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    868..892
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(892..911)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(893..911)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tttgttagca gccggatccg ctcttaatcc gcgcgcggtg cgatgtagaa atcaccgtag
       61 ccttcctgcg gcagtttaaa acgcagtttc agcggaatct ggctaccgaa gtacagttcc
      121 atagaatcgc tcgcgcgacg cattttcgtg gtatttgcaa cgtattccat gccatagcta
      181 ctagacacat cggcaccaga cgcttccagc agcgtgccgt tatcggtaga cagttcaact
      241 ttcgcggtgc tcagatcacc gatcacttcg aagtacagtt tgttttcttt gctatggatg
      301 ttcagcactt cgcccagatc actcagttca tcgataatat cggcaaacgt aatggtcagc
      361 agctgcgctt tgaacggaaa ttccagatta acactcggcg gctgcgtaga ttccacctga
      421 atcagcggca gttcaaaact gcgggtaaat tcaccatcaa acgtcagggt cagtttagat
      481 tcattgctac tcaggatcag ggtatcatct ttcagaacac gtttcagaat atcgttcaca
      541 tcttccagtt tgaaaccgat gatttctttt tcctggctaa cttcgaagcc ttcaaagtaa
      601 gagctcggca ggaagatatc cagaaaaacc acgcggctcg gatcgatacc actaacacga
      661 atgccttctt tcgtcacaat aaagttggct tcgctcagga aatcgcccac ggtacgcagg
      721 atgtaactga aagaaactgc atcgatcact ttggctttca tcatatggct gccgcgcggc
      781 accaggccgc tgctgtgatg atgatgatga tggctgctgc ccatggtata tctccttctt
      841 aaagttaaac aaaattattt ctagagggga attgttatcc gctcacaatt cccctatagt
      901 gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct acgccgga
//
