LOCUS       sequence-117580-        1040 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13N-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..1040
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(859..892)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    893..918
                     /label=SA
                     /note="splice acceptor site"
     CDS             942..995
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgattacgcc gcggccgcag caggagaatc atgccaatgg gccaatatac attctgaccc
       61 acagtttcat aataaaataa aatggttgtg gttgtaagcc actatgtttc agagtggttt
      121 gttacacagc aataaataac taatatagta ggcataccat caagtccaaa gtaggtagag
      181 aagaatgtaa atagcagagc aaaacagcat gactggtggc tgggaggctt aaaactggga
      241 caggatcaga gtcatgaaag aagtcaaaga aatggttcag aagtaaggct gagactgact
      301 tacaaaagct gaaagtccct ttaagttggt gtttggtgca ttggcagggg caggtatggt
      361 gacttaaaag agccatgctc aacaagatca agcacaacac aatcacgggt caccccagca
      421 gaccttagcg agtctagcca tttctttggt ggtggtcaca gtcatgcttc agcccagttt
      481 ccacttggac aaatggtaca tattttcaat gagatgaaaa ttaagataca atccatgtgc
      541 tcagagagtg atcacagctc tgactaaaca ctgtgcccca aagtgttgag gaattgggaa
      601 aacctagctg agttagtggt ctcttttctg ttacaataaa gctcataatg aaaattagcc
      661 ttctttgttc ttccccaagt ctttctttct agacgaaact actttcaact gttttaactt
      721 ccttactgtt aacttccata tttctagata gtatggtcag actgttattt cttgactttt
      781 aaatgtaaga tattatctac tgacttcctt ctatgtaaga tgaggattga gctctcttac
      841 ccttctccca gcggccgcat aacttcgtat agcatacatt atacgaagtt atctgacctc
      901 ttctcttcct cccacagggc ctcgagagat ctggcagcgg agagggcaga ggaagtcttc
      961 taacatgcgg tgacgtggag gagaatcccg gccctaggat tgaacaagat ggattgcacg
     1021 caggttctcc ggccgcttgg
//
