LOCUS       sequence-117583-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13N-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    209..242
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
       61 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      121 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      181 gattactatt aataactagc ctgaattgat aacttcgtat aaagtatcct atacgaagtt
      241 atgtcgaccg atcgtcgcga gaattcggcg cgccaatatt ctcactgact ccgtcctgga
      301 gttggatgag agataatggc cttacgttgt gccaggggag ggtcgggctg gatttagcaa
      361 gatttacctt ctccaaagag cggtgctgca gtggcacagc tgcccacgga ggtggggggg
      421 tcaccgtccc tggaggtgat gaagaactgt ggggatgtgg cactgaggga catggccagt
      481 gggcacggtg ggtgggttgg ggttggtctt ggggatcttg gagggctttt ccagccttca
      541 tgatttgacg attgtatgaa catctacatg gcaattctcc agctgcctgt cccagtccta
      601 ctgacccagc tgtatctctc caggcaagct cttccacccc ttctgcttgc atccagacac
      661 catcaaacat gcaggctcag acacagggac cagcagtgtc tgtggccttt ttgtgctcct
      721 ctccatgctg ggttttaact tgctctttgt ccttctatcc tatcttctta tccttaaggc
      781 tgttctgaac gctgt
//
