LOCUS       sequence-117589-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for transposon tagging with a fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Sheridan DL, Hughes TE
  TITLE     A faster way to make GFP-based biosensors: two new transposons for 
            creating multicolored libraries of fluorescent fusion proteins.
  JOURNAL   BMC Biotechnol. 2004 Aug 18;4:17.
  PUBMED    15317651
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2004-08-18"; volume: "4"; pages: "17"
COMMENT     Sequence Label: pBNJ24.6
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..43
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(113..132)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(114..132)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(139..156)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(139..155)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(148..170)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             293..595
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     474..493
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gctcctcgcc cttgctggcg cgccagatgt gtataagaga cagaagggcg aattctgcag
       61 atatccatca cactggcggc cgctcgagca tgcatctaga gggcccaatt cgccctatag
      121 tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
      181 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
      241 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctatacgtac ggcagtttaa
      301 ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac agagtgatat
      361 tattgacacg ccggggcgac ggatggtgat ccccctggcc agtgcacgtc tgctgtcaga
      421 taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct ggcgcatgat
      481 gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg ctgatctcag
      541 ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa tataaatgtc
      601 aggcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttcacgtaga aagccagtcc
      661 gcagaaacgg tgctgacccc ggatgaatgt cagctactgg gctatctgga caagggaaaa
      721 cgcaagcgca aagagaaagc aggtagcttg cagtgggctt acatggc
//
