LOCUS       sequence-117591-         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the E105C/L171C mutant of Sulfolobus solfataricus PCNA2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 979)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA2 E105C/L171C
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(767..784)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(794..811)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             830..852
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    867..891
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(891..910)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(892..910)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ttgttagcag ccggatccgc tcttaatccg cgcgcggtgc gatgtagaaa tcaccgtagc
       61 cttcctgcgg cagtttaaaa cgcagtttca gcggaatctg gctaccgaag tacagttcca
      121 tagaatcgct cgcgcgacgc attttcgtgg tatttgcaac gtattccatg ccatagctac
      181 tagacacatc ggcaccagac gcttccagca gcgtgccgtt atcggtagac agttcaactt
      241 tcgcggtgct gcaatcaccg atcacttcga agtacagttt gttttctttg ctatggatgt
      301 tcagcacttc gcccagatca ctcagttcat cgataatatc ggcaaacgta atggtcagca
      361 gctgcgcttt gaacggaaat tccagattaa cactcggcgg ctgcgtagat tccacctgaa
      421 tcagcggcag ttcaaaactg cgggtaaagc aaccatcaaa cgtcagggtc agtttagatt
      481 cattgctact caggatcagg gtatcatctt tcagaacacg tttcagaata tcgttcacat
      541 cttccagttt gaaaccgatg atttcttttt cctggctaac ttcgaagcct tcaaagtaag
      601 agctcggcag gaagatatcc agaaaaacca cgcggctcgg atcgatacca ctaacacgaa
      661 tgccttcttt cgtcacaata aagttggctt cgctcaggaa atcgcccacg gtacgcagga
      721 tgtaactgaa agaaactgca tcgatcactt tggctttcat catatggctg ccgcgcggca
      781 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta
      841 aagttaaaca aaattatttc tagaggggaa ttgttatccg ctcacaattc ccctatagtg
      901 agtcgtatta atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg
      961 gcatcaccgg cgccacagg
//
