LOCUS       sequence-117597-         501 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the R112C mutant of Sulfolobus solfataricus PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 501)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA3 R112C
FEATURES             Location/Qualifiers
     source          1..501
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gcggcctggt gccgcgcggc agccatatga tctacctgaa atctttcgaa cgtaacatcc
       61 gcctgatcaa tatgaaagtg gtttacgatg atgttcgtgt gctgaaagat attatccagg
      121 cgctggcccg cctggttgat gaagccgtgc tgaaattcaa acaggatagc gttgaactgg
      181 tggcactgga tcgtgcgcat attagcctga tctctgttaa cctgccgcgc gaaatgttca
      241 aagaatacga tgtgaacgat gaatttaaat tcggtttcaa cacccagtac ctgatgaaaa
      301 tcctgaaagt tgcaaaacgt aaagaagcca ttgaaatcgc aagtgaaagc ccggatagtg
      361 tgattatcaa cattatcggc agcaccaatc gtgaatttaa cgtttgcaat ctggaagtgt
      421 ctgaacagga aattccggaa atcaacctgc agttcgatat tagtgcgacg atcagctctg
      481 atggttttaa aagtgccatc a
//
