LOCUS       sequence-117598-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the R112C mutant of Sulfolobus solfataricus PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA3 R112C
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(807..824)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(834..851)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             870..892
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    907..931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgttagcag ccggatccgc tcttcacact ttcggcgcca gcaggtacgt aactttgcca
       61 ccgccttcca tattgaaaaa cagctgcagc ggtttctggt tgccgaagct gattttcacg
      121 taatcactca gtttcgtcag agacagaaca tcatccaggt attccgcact gtaagaattt
      181 ttgctttctt tactaaattc cagatcctgc agaccgccgg tatctttaga aaattccact
      241 tcaacttcgc tttcgccttc tgctttgatc agaatgcgat cttcgtgacc ttccacaacc
      301 acattatccg tcacggtaga aacttcgctg atggcacttt taaaaccatc agagctgatc
      361 gtcgcactaa tatcgaactg caggttgatt tccggaattt cctgttcaga cacttccaga
      421 ttgcaaacgt taaattcacg attggtgctg ccgataatgt tgataatcac actatccggg
      481 ctttcacttg cgatttcaat ggcttcttta cgttttgcaa ctttcaggat tttcatcagg
      541 tactgggtgt tgaaaccgaa tttaaattca tcgttcacat cgtattcttt gaacatttcg
      601 cgcggcaggt taacagagat caggctaata tgcgcacgat ccagtgccac cagttcaacg
      661 ctatcctgtt tgaatttcag cacggcttca tcaaccaggc gggccagcgc ctggataata
      721 tctttcagca cacgaacatc atcgtaaacc actttcatat tgatcaggcg gatgttacgt
      781 tcgaaagatt tcaggtagat catatggctg ccgcgcggca ccaggccgct gctgtgatga
      841 tgatgatgat ggctgctgcc catggtatat ctccttctta aagttaaaca aaattatttc
      901 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcg
//
