LOCUS       sequence-117608-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the R112C/T180C mutant of Sulfolobus solfataricus PCNA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Hirakawa H, Kakitani A, Nagamune T
  TITLE     Introduction of selective intersubunit disulfide bonds into 
            self-assembly protein scaffold to enhance an artificial multienzyme 
            complex's activity.
  JOURNAL   Biotechnol Bioeng. 2013 Jul;110(7):1858-64. doi: 10.1002/bit.24861. 
            Epub 2013 Feb 22.
  PUBMED    23404255
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/bit.24861";
            journalName: "Biotechnol Bioeng"; date: "2013-07"; volume: "110"; 
            issue: "7"; pages: "1858-64"
COMMENT     Sequence Label: pET15b+PCNA3 R112C/T180C
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(806..823)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(833..850)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             869..891
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    910..926
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ttgtagcagc cggatccgct cttcacactt tcggcgccag caggtacgta actttgccac
       61 cgccttccat attgaaaaac agctgcagcg gtttctggtt gccgaagctg attttcacgt
      121 aatcactcag tttcgtcaga gacagaacat catccaggta ttccgcactg taagaatttt
      181 tgctttcttt actaaattcc agatcctgca gaccgccgca atctttagaa aattccactt
      241 caacttcgct ttcgccttct gctttgatca gaatgcgatc ttcgtgacct tccacaacca
      301 cattatccgt cacggtagaa acttcgctga tggcactttt aaaaccatca gagctgatcg
      361 tcgcactaat atcgaactgc aggttgattt ccggaatttc ctgttcagac acttccagat
      421 tgcaaacgtt aaattcacga ttggtgctgc cgataatgtt gataatcaca ctatccgggc
      481 tttcacttgc gatttcaatg gcttctttac gttttgcaac tttcaggatt ttcatcaggt
      541 actgggtgtt gaaaccgaat ttaaattcat cgttcacatc gtattctttg aacatttcgc
      601 gcggcaggtt aacagagatc aggctaatat gcgcacgatc cagtgccacc agttcaacgc
      661 tatcctgttt gaatttcagc acggcttcat caaccaggcg ggccagcgcc tggataatat
      721 ctttcagcac acgaacatca tcgtaaacca ctttcatatt gatcaggcgg atgttacgtt
      781 cgaaagattt caggtagatc atatggctgc cgcgcggcac caggccgctg ctgtgatgat
      841 gatgatgatg gctgctgccc atggtatatc tccttcttaa agttaaacaa aattatttct
      901 agaggggaat tgttatccgc tcacaat
//
