LOCUS       sequence-117615-         775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Parent vector used for inserting cis-regulatory elements (CREs) for 
            CRE-seq in ES cells. Uses the Pou5f1 basal promoter and dsRed gene..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Fiore C, Cohen BA
  TITLE     Interactions between pluripotency factors specify cis-regulation in 
            embryonic stem cells.
  JOURNAL   Genome Res. 2016 Jun;26(6):778-86. doi: 10.1101/gr.200733.115. Epub 
            2016 Apr 15.
  PUBMED    27197208
REFERENCE   2  (bases 1 to 775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.200733.115"; journalName: "Genome Res"; date: "2016-06";
            volume: "26"; issue: "6"; pages: "778-86"
COMMENT     Sequence Label: pGL-Pou
FEATURES             Location/Qualifiers
     source          1..775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(22..699)
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(84..107)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(282..301)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     499..519
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ctcggctcga gtctagaatt actactggaa caggtggtgg cgggcctcgg cgcgctcgta
       61 ctgctccacc acggtgtagt cctcgttgtg ggaggtgatg tccagcttgg agtccacgta
      121 gtagtagccg ggcagcttca cgggcttctt ggccatgtag attgacttga actccaccag
      181 gtagtggccg ccgcccttca gcttcagcgc cttgtggatc tcgcccttca gcacgccgtc
      241 gcgggggtac aggcgctcgg tggagggctc ccagcccaga gtcttcttct gcattacggg
      301 gccgtcggag gggaagttca cgccgatgaa cttcacgtgg tagatgaagg tgccgtcctg
      361 cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcacgcgctc
      421 ccacttgaag ccctcgggga aggacagctt cttgtagtcg gggatgtcgg cggggtgctt
      481 cacgtacacc ttggagccgt actggaactg gggggacagg atgtcccagg cgaagggcag
      541 ggggccgccc ttggtcacct gcagcttggc ggtctgggtg ccctcgtagg gcttgccctc
      601 gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
      661 gcgcatgaag ggcttgatga cgttctcagt gctatccatg gggaaggtgg gcaccccgag
      721 ccgggggcct ggtggaaaga cggctcacct agggacggtt tcacctctcc ctccc
//
