LOCUS       sequence-117616-         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  EGFP-based homologous recombination-dependent reporter for genome 
            editing with TALENs or CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Flemr M, Buhler M
  TITLE     Single-Step Generation of Conditional Knockout Mouse Embryonic Stem 
            Cells.
  JOURNAL   Cell Rep. 2015 Jul 28;12(4):709-16. doi: 
            10.1016/j.celrep.2015.06.051. Epub 2015 Jul 16.
  PUBMED    26190102
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.06.051"; journalName: "Cell Rep"; date: 
            "2015-07-28- 28"; volume: "12"; issue: "4"; pages: "709-16"
COMMENT     Sequence Label: pMB1609_pRR-EGFP
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(20..730,731..733))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(20..730,731..733))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(62..83)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     411..430
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     670..691
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 cggccgctta gagtccggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
       61 tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg
      121 actgggtgct caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt
      181 tctgctggta gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga
      241 agttcacctt gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt
      301 agttgtactc cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca
      361 gctcgatgcg gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt
      421 tgccgtcgtc cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact
      481 tgaagaagtc gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg
      541 tcagggtggt cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact
      601 tcagggtcag cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt
      661 ggccgtttac gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct
      721 cgcccttgct cacttagacg tcaagagctc tcattagtag gtcagg
//
