LOCUS       sequence-117618-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inntag 5: a small self-structured epitope aimed at innocuous protein
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R,
            Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M
  TITLE     Inntags: small self-structured epitopes for innocuous protein 
            tagging.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556.
  PUBMED    26322837
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556."
COMMENT     Sequence Label: pBS-IT5
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(310..326)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(310..326)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(363..383)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(363..381)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(402..418)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(402..418)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(415..437)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    426..442
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(450..456,457..474,475..480))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    495..516
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(633..650)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ttcttccggg gcataggtgg cgccaatggt atatttttcc ggcaccacat catcaaacac
       61 gtttttcatg cctttttcgg tcagaaagcg aaagttaaac gggccctgca gcggttcttc
      121 gctatcaaag gtccacacgc cgccttcgcc tttggtcatg gccacccatt catcgctgcc
      181 atgttcgcgc agttccactt cggccatggt atcgccttca tatttcacca gcacggccag
      241 atgtttttcg ttgctgcctt tttccacggt aaaggtcact ttcggcacca gaattcctgc
      301 agcccggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
      361 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg
      421 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      481 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      541 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
      601 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
      661 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
      721 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaa
//
