LOCUS       sequence-117620-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inntag 6: a small self-structured epitope aimed at innocuous protein
            tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Georgieva MV, Yahya G, Codo L, Ortiz R, Teixido L, Claros J, Jara R,
            Jara M, Iborra A, Gelpi JL, Gallego C, Orozco M, Aldea M
  TITLE     Inntags: small self-structured epitopes for innocuous protein 
            tagging.
  JOURNAL   Nat Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556.
  PUBMED    26322837
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Aug 31. doi: 10.1038/nmeth.3556."
COMMENT     Sequence Label: pBS-IT6
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(152..168)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(152..168)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(205..225)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(205..223)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(244..260)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(244..260)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(257..279)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(292..298,299..316,317..322))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    337..358
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(475..492)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(726..745)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gatctttgca gttgctctgg cagttatgat ccgggctgca atattcatcg gtgctgccgc
       61 accagcccca ctggctgcag cacaggttat ccgggcacag tttgccgccg gcctggcggc
      121 cgcactgttc cagaattcct gcagcccggg ggatccacta gttctagagc ggccgccacc
      181 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
      241 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
      301 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
      361 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
      421 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
      481 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      541 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
      601 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
      661 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
      721 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
      781 cctgccgctt accggatacc tgtccgcctt tctcccttcg g
//
