LOCUS       sequence-117623-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13N-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    123..347
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(348..381)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
       61 ctatcgcctt cttgacgagt tcttctaaca cctggtgcat gacccgcaag cccggtgcct
      121 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      181 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
      241 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
      301 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggata acttcgtata
      361 gcatacatta tacgaagtta tgagctcacg gggacagccc ccccccaaag cccccaggga
      421 tgtaattacg tccctccccc gctagggggc agcagcgagc cgcccggggc tccgctccgg
      481 tccggcgctc cccccgcatc cccgagccgg cagcgtgcgg ggacagcccg ggcacgggga
      541 aggtggcacg ggatcgcttt cctctgaacg cttctcgctg ctctttgagc ctgcagacac
      601 ctggggggat acggggaaaa agctttaggc tgaaagagag atttagaatg acagaatcat
      661 agaacggcct gggttgcaaa ggagcacagt gctcatccag atccaacccc ctgctatgtg
      721 cagggtcatc aaccagcagc ccaggctgcc cagagccaca tccagcctgg ccttgaatgc
      781 ctgcagggat ggggcatcca cagcctcctt gggcaacctg ttcagtgcgt caccaccctc
      841 tggg
//
