LOCUS       sequence-117624-         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP,
            compatible with pZT-C13 TALENs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pC13N-iCAG.copGFP
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..805)
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     primer_bind     complement(866..885)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga
       61 attcacgcgt ttagcgagat ccggtggagc cgggtccggc ggtgccgtcc acggcagaat
      121 tggacgactg agcgcgggat ctggcgaagg cgatgggggt cttgaaggcg tgctggtact
      181 ccacgatgcc cagctcggtg ttgctgtgca gctcctccac gcggcggaag gcgaacatgg
      241 ggcccccgtt ctgcaggatg ctggggtgga tggcgctctt gaagtgcatg tggctgtcca
      301 ccacgaagct gtagtagccg ccgtcgcgca ggctgaaggt gcgggcgaag ctgcccacca
      361 gcacgttatc gcccatgggg tgcaggtgct ccacggtggc gttgctgcgg atgatcttgt
      421 cggtgaagat cacgctgtcc tcggggaagc cggtgcccac caccttgaag tcgccgatca
      481 cgcggccggc ctcgtagcgg tagctgaagc tcacgtgcag cacgccgccg tcctcgtact
      541 tctcgatgcg ggtgttggtg tagccgccgt tgttgatggc gtgcaggaag gggttctcgt
      601 agccgctggg gtaggtgccg aagtggtaga agccgtagcc catcacgtgg ctcagcaggt
      661 aggggctgaa ggtcagggcg cctttggtgc tcttcatctt gttggtcatg cggccctgct
      721 tgggggtgcc ctctccgccg cccaccagct cgaactccac gccgttcagg gtgccggtga
      781 tgcggcactc gatctccatg gcgggcaggc cgctctcgtc gctctccatg gttgtacaca
      841 attctttgcc aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga
//
