LOCUS       sequence-117633-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: ADRA1D-Tango
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      715..724
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgac ttttagggac ttgcttagcg tctcctttga ggggccccga
      301 ccagacagca gcgccggagg aagcagtgct gggggggggg gtgggtccgc cggaggcgct
      361 gctccctccg aggggccggc ggtgggaggt gtgccagggg gagccggcgg cgggggtgga
      421 gtcgtgggcg ctggctcagg agaggataat aggtcttccg ccggggaacc agggagcgcc
      481 ggggctgggg gtgatgtgaa cgggactgcc gccgtaggcg gtctggttgt gagtgcacag
      541 ggtgtagggg tgggcgtatt tctcgcagca ttcatcctga tggctgttgc cggcaacctg
      601 ctggttatct tgagtgtggc ttgcaatcgg caccttcaga ctgtgacaaa ctacttcatc
      661 gtgaacctgg ccgtcgccga tcttctcctg tcagccaccg tgctgccctt ttctgccacc
      721 atggaggtac ttggcttctg ggctttcggt cgagcctttt gcgatgtatg ggccgccgtg
      781 gatgtcctgt gttgtactgc aagcattctg agtttgtgta ctatttccgt cgaccgctat
      841 gtgggcgtga gacactctct caagtatcct gcaattatga ccgaacgaaa agcggcagcc
      901 atcctggctc tgctgt
//
